Anticorps Polyclonal de lapin anti-PAX6

• Phare
• Validé par KD/KO

PAX6 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

52

Avis (3)

N° de cat : 12323-1-AP

Synonymes

AN, AN2, Aniridia type II protein, D11S812E, MGDA, Oculorhombin, paired box 6, Paired box protein Pax 6, PAX6, WAGR

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Galerie de données de validation

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  WB analysis using 12323-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12323-1-AP (PAX6 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of human stomach using 12323-1-AP

  human stomach tissue were subjected to SDS PAGE followed by western blot with 12323-1-AP (PAX6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse embryo using 12323-1-AP

  mouse embryo tissue were subjected to SDS PAGE followed by western blot with 12323-1-AP (PAX6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 12323-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12323-1-AP (PAX6 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 12323-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12323-1-AP (PAX6 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse eye using 12323-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 12323-1-AP (PAX6 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0).

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  IHC staining of mouse eye using 12323-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 12323-1-AP (PAX6 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0).

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  IHC staining of human retinoblastoma using 12323-1-AP

  Immunohistochemical analysis of paraffin-embedded human retinoblastoma tissue slide using 12323-1-AP (PAX6 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0).

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  IHC staining of human retinoblastoma using 12323-1-AP

  Immunohistochemical analysis of paraffin-embedded human retinoblastoma tissue slide using 12323-1-AP (PAX6 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0).

• 

  IHC staining of mouse brain using 12323-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12323-1-AP (PAX6 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 12323-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12323-1-AP (PAX6 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse embryo using 12323-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 12323-1-AP (PAX6 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse embryo using 12323-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 12323-1-AP (PAX6 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of Retinal organoids using 12323-1-AP

  Retinal organoids (day 30) generated from human induced pluripotent stem cells (iPSCs) and fixed with 4% PFA. Stained for PAX6 with 12323-1-AP at 1:600 (green), and SOX2 with 11064-1-AP at 1:200 (red), Nuclear stain DAPI (blue). Scale bar = 20 µm. Data generated by Alessandro Bellapianta at Johannes Kepler Universitat, Austria.

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  IF Staining of Retinal organoids using 12323-1-AP

  Retinal organoids (day 30) generated from human induced pluripotent stem cells (iPSCs) and fixed with 4% PFA. Stained for Tubulin beta 3/TUJ1 using 66375-1-Ig at 1:500 dilution (green) and PAX6 (12323-1-AP) at 1:500. Nuclear stain DAPI (blue). Scale bar = 50 µm. Data generated by Alessandro Bellapianta at Johannes Kepler Universitat, Austria.

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  IF Staining of iPS cells using 12323-1-AP

  Immunofluorescent staining of PAX6 (12323-1-AP, 1:250 dilution) with 4% PFA fixed control human induced pluripotent stem cells (hiPSC) derived neuronal precursor cells (NPCs). (Green: PAX6; Blue: DAPI). Provided by BioTalentum Ltd., Hungary.

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  FC experiment of SH-SY5Y using 12323-1-AP

  1X10^6 SH-SY5Y cells were stained with 0.2ug PAX6 antibody (12323-1-AP, red) and control antibody (blue). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100.

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Applications testées

• Résultats positifs en WB: cellules Y79, tissu cérébral de rat, tissu d'estomac humain, tissu embryonnaire de souris
• Résultats positifs en IHC: tissu cérébral de souris, tissu de rétinoblastome humain, tissu embryonnaire de souris, tissu oculaire de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: Retinal organoids, cellules iPS
• Résultats positifs en cytométrie: cellules SH-SY5Y

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:10-1:100
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 14 publications below
• IHC: See 6 publications below
• IF: See 37 publications below

Informations sur le produit

12323-1-AP cible PAX6 dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PAX6 Protéine recombinante Ag2984
• Nom complet: paired box 6
• Masse moléculaire calculée: 47 kDa
• Poids moléculaire observé: 47 kDa
• Numéro d’acquisition GenBank: BC011953
• Symbole du gène: PAX6
• Identification du gène (NCBI): 5080
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Background

Paired-box 6 (Pax6) is a highly conserved nuclear transcription factor that is key in neural tissue development, particularly eye morphogenesis.

What is the molecular weight of Pax6?

46.6 kDa. Pax6 is composed of 422 amino acids and contains a paired domain and a homeodomain.

What is the role of Pax6 in development?

In embryos, Pax6 is expressed in the developing central nervous system (CNS), eye, nose, and also in the pancreas. As a transcription factor, Pax6 is one of the earliest genes expressed in the eye and regulates the expression of many genes required for retinal and olfactory development (PMID: 10461206). The broad expression of Pax6 in the neuroectoderm, which develops to the CNS, makes it a useful marker for neural stem cells (PMID: 20621053).

The homology of Pax6 to the Drosophila gene eyeless and the mouse gene Small eye highlights its importance across a wide spectrum of organisms. In model organisms such as flies or mice, a dramatic phenotype can be seen. Mutations in eyeless lead to ectopic eye development and mice with heterozygote small eye mutations do not develop eyes or nasal cavities, and homozygote small eye mice do not survive (PMID: 7892602; PMID 8875254).

What is the role of Pax6 in eye disease?

Due to its key role as a master regulator in eye development, mutations in Pax6 have been associated with many different eye diseases. In humans, premature stop signals that lead to the production of a truncated protein have been found to cause aniridia, the absence of an iris (PMID: 19898691). Single amino acid mutations in Pax6 may lead to Peters' anomaly, which causes thinning and clouding of the cornea (PMID: 8162071).

Publications

Species	Application	Title
	IF	Cell Stem Cell; Engineering Genetic Predisposition in Human Neuroepithelial Stem Cells Recapitulates Medulloblastoma Tumorigenesis.; Authors - Miller Huang
mouse	WB	Cell Stem Cell; Chemical-induced chromatin remodeling reprograms mouse ESCs to totipotent-like stem cells.; Authors - Mingzhu Yang
mouse	WB	Cell Metab; Mitochondrial Dynamics Is Critical for the Full Pluripotency and Embryonic Developmental Potential of Pluripotent Stem Cells.; Authors - Xiuying Zhong
human	WB,IF	Cell Rep Med; End binding-3 inhibitor activates regenerative program in age-related macular degeneration; Authors - Quinn Lee
mouse	WB	Nat Commun; ADNP promotes neural differentiation by modulating Wnt/β-catenin signaling.; Authors - Xiaoyun Sun
human	IF	Cell Rep; Astrocytes mediate cell non-autonomous correction of aberrant firing in human FXS neurons; Authors - Shreya Das Sharma

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Ryszard (Verified Customer) (01-19-2024)

Antibody Pax-6 Rabbit works very well on whole mount mESC-derived cerebral organoids.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1/500
• Cell Tissue Type: mESC-derived Cerebral Organoids

PAX6 Antibody Immunofluorescence validation (1/500 dilution) in mESC-derived Cerebral Organoids (Cat no:12323-1-AP)

FH

Julie (Verified Customer) (01-18-2021)

This antibody works very well for immunofluorescence on human brain.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Human

FH

Rachel (Verified Customer) (02-01-2019)

Worked well and Proteintech employees were very helpful during the ordering process.

• Applications: Other,
• Cell Tissue Type: Epithelial cells

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