Anticorps Monoclonal anti-PAX6

PAX6 Monoclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

1

CloneNo.

2C5A1

N° de cat : 67529-1-Ig

Synonymes

AN, AN2, Aniridia type II protein, D11S812E, MGDA, Oculorhombin, paired box 6, Paired box protein Pax 6, PAX6, WAGR

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Galerie de données de validation

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  WB analysis of rat retina using 67529-1-Ig

  rat retina tissue were subjected to SDS PAGE followed by western blot with 67529-1-Ig (PAX6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 67529-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67529-1-Ig (PAX6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 67529-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67529-1-Ig (PAX6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of JAR using 67529-1-Ig

  JAR cells were subjected to SDS PAGE followed by western blot with 67529-1-Ig (PAX6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of hTERT-RPE1 using 67529-1-Ig

  hTERT-RPE1 cells were subjected to SDS PAGE followed by western blot with 67529-1-Ig (PAX6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 67529-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67529-1-Ig (PAX6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Neuro-2a using 67529-1-Ig

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 67529-1-Ig (PAX6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse eye using 67529-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 67529-1-Ig (PAX6 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse eye using 67529-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 67529-1-Ig (PAX6 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 67529-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67529-1-Ig (PAX6 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 67529-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67529-1-Ig (PAX6 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 67529-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67529-1-Ig (PAX6 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 67529-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67529-1-Ig (PAX6 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu rétinien de rat, cellules HeLa, cellules hTERT-RPE1, cellules JAR, cellules K-562, cellules Neuro-2a
• Résultats positifs en IHC: tissu oculaire de souris, tissu cérébral de rat, tissu cérébral de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 1 publications below

Informations sur le produit

67529-1-Ig cible PAX6 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PAX6 Protéine recombinante Ag29977
• Nom complet: paired box 6
• Masse moléculaire calculée: 47 kDa
• Poids moléculaire observé: 47 kDa
• Numéro d’acquisition GenBank: BC011953
• Symbole du gène: PAX6
• Identification du gène (NCBI): 5080
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20 ℃. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PAX6, a paired domain and homeodomain-containing transcription factor. Interaction with TRIM11 leads to ubiquitination of PAX6 and its proteasomal degradation. PAX6 is one of the earliest genes expressed in the eye field and considered a master control gene for retinal and eye development. PAX6 also regulates the development of the olfactory, central nervous systems, pituitary, and pancreas. PAX6 mutations can cause complex ocular disorders such as aniridia and Peter's anomaly.

Publications

Species	Application	Title
human	IF	Anal Bioanal Chem; Simultaneous and quantitative monitoring transcription factors in human embryonic stem cell differentiation using mass spectrometry-based targeted proteomics.; Authors - Mengying Xu