Anticorps Recombinant de lapin anti-PAX6

PAX6 Recombinant Antibody for FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

FC (Intra)

Conjugaison

PE Fluorescent Dye

CloneNo.

241521G1

N° de cat : PE-84345-5

Synonymes

241521G1, paired box 6, Paired box protein Pax 6, Paired box protein Pax-6, PAX 6

---

Galerie de données de validation

• 

  FC experiment of U-251 using PE-84345-5

  1x10^6 U-251 cells were intracellularly stained with 5 ul PE Pax6 Recombinant Antibody (PE-84345-5, Clone:241521G1)(red), or PE Rabbit IgG Isotype Control RecAb (PE-98136, Clone: 240953C9) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

• 

  FC experiment of HeLa using PE-84345-5

  1x10^6 HeLa cells were intracellularly stained with 5 ul PE Pax6 Recombinant Antibody (PE-84345-5, Clone:241521G1)(red), or PE Rabbit IgG Isotype Control RecAb (PE-98136, Clone: 240953C9) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

---

Applications testées

• Résultats positifs en FC (Intra): cellules U-251, cellules HeLa

Dilution recommandée

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 5 ul per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

PE-84345-5 cible PAX6 dans les applications de FC (Intra) et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: PAX6 Protéine recombinante Ag2984
• Nom complet: paired box 6
• Masse moléculaire calculée: 47 kDa
• Poids moléculaire observé: 47 kDa
• Numéro d’acquisition GenBank: BC011953
• Symbole du gène: PAX6
• Identification du gène (NCBI): 5080
• Conjugaison: PE Fluorescent Dye
• Excitation/Emission maxima wavelengths: 496 nm, 565 nm / 578 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. 20ul contiennent 0,1% de BSA.

Informations générales

PAX6, a paired domain and homeodomain-containing transcription factor. Interaction with TRIM11 leads to ubiquitination of PAX6 and its proteasomal degradation. PAX6 is one of the earliest genes expressed in the eye field and considered a master control gene for retinal and eye development. PAX6 also regulates the development of the olfactory, central nervous systems, pituitary, and pancreas. PAX6 mutations can cause complex ocular disorders such as aniridia and Peter's anomaly.

{{ptg:RelatedPrimaryAntibodies}}