Anticorps Monoclonal anti-PAX8

PAX8 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugaison

Non conjugué

Publications(9)

CloneNo.

4H7B3

Avis (2)

N° de cat : 60145-4-Ig

Synonymes

PAX-8, PAX 8, Paired box protein Pax-8, Paired box protein Pax 8, paired box 8

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Galerie de données de validation

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  WB analysis of SKOV-3 using 60145-4-Ig

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 60145-4-Ig (PAX8 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human thyroid gland using 60145-4-Ig

  human thyroid gland tissue were subjected to SDS PAGE followed by western blot with 60145-4-Ig (PAX8 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human ovary tumor using 60145-4-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60145-4-Ig (PAX8 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 60145-4-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60145-4-Ig (PAX8 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary cancer using 60145-4-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 60145-4-Ig (PAX8 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 60145-4-Ig

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 60145-4-Ig (PAX8 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 60145-4-Ig

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 60145-4-Ig (PAX8 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 60145-4-Ig

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 60145-4-Ig (PAX8 antibody) at dilution of 1:15000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 60145-4-Ig

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 60145-4-Ig (PAX8 antibody) at dilution of 1:15000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 60145-4-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60145-4-Ig (PAX8 antibody) at dilution of 1:12800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 60145-4-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60145-4-Ig (PAX8 antibody) at dilution of 1:12800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 60145-4-Ig

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 60145-4-Ig (PAX8 antibody) at dilution of 1:12800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 60145-4-Ig

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 60145-4-Ig (PAX8 antibody) at dilution of 1:12800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma tissue using 60145-4-Ig

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using DA00001(PAX8 antibody) at dilution of 1:6400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma tissue using 60145-4-Ig

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide usingDA00001 (PAX8 antibody) at dilution of 1:6400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse kidney using 60145-4-Ig

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using PAX8 antibody (60145-4-Ig, Clone: 4H7B3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of SKOV-3 using 60145-4-Ig

  Confocal immunofluorescent analysis of (4% PFA) fixed SKOV-3 cells using 60145-4-Ig (PAX8 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). Phalloidin stains F-actin (Red).

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Applications testées

• Résultats positifs en WB: cellules SKOV-3, tissu de glande thyroïdienne humain
• Résultats positifs en IHC: tissu de tumeur ovarienne humain, tissu d'amygdalite humain, tissu de cancer de la thyroïde humain, tissu rénal de rat, tissu rénal de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu rénal de souris,
• Résultats positifs en IF/ICC: cellules SKOV-3,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:5000-1:20000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 6 publications below
• IF: See 3 publications below

Informations sur le produit

60145-4-Ig cible PAX8 dans les applications de WB, IHC, IF/ICC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PAX8 Protéine recombinante Ag0306
• Nom complet: paired box 8
• Masse moléculaire calculée: 48 kDa
• Poids moléculaire observé: 48 kDa
• Numéro d’acquisition GenBank: BC001060
• Symbole du gène: PAX8
• Identification du gène (NCBI): 7849
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PAX8 is a member of the paired box (PAX) family of transcription factors, typically containing a paired box domain and a paired-type homeodomain. It is expressed during organogenesis of the thyroid gland, kidney and Mullerian system. It is thought to regulate the expression of Wilms tumor suppressor (WT1) gene and mutations in PAX8 have been associated with Wilms tumor cells, thyroid and ovarian carcinomas. PAX8 is a useful marker in distinguishing ovarian carcinomas from mammary carcinomas (PMID: 18724243). PAX8 is expressed in a high percentage of ovarian serous, endometrioid, and clear cell carcinomas, but only rarely in primary ovarian mucinous adenocarcinomas.

PAX8 has 5 isoforms with calculated MW 31kd, 34kd, 41kd, 43kd and 48kd. And there is a 60kd band corresponding to the electrophoretic mobility of PAX8 (PMID:15650356).

Publications

Species	Application	Title
human	IF	Dev Cell; Identification of a core transcriptional program driving the human renal mesenchymal-to-epithelial transition; Authors - John-Poul Ng-Blichfeldt
human	IHC	Mod Pathol; Xp11.2 translocation renal cell carcinoma with NONO-TFE3 gene fusion: morphology, prognosis, and potential pitfall in detecting TFE3 gene rearrangement.; Authors - Qiu-Yuan Xia
human	IF	Stem Cell Res Ther; Genetic correction of induced pluripotent stem cells from a DFNA36 patient results in morphologic and functional recovery of derived hair cell-like cells; Authors - Yi Luo
mouse	IHC,IF	Front Cell Dev Biol; Lgr4 Regulates Oviductal Epithelial Secretion Through the WNT Signaling Pathway; Authors - Xue Tan
human	IHC	Sci Rep; Differential epithelial and stromal LGR5 expression in ovarian carcinogenesis.; Authors - Hyesung Kim
human	IHC	Hum Pathol; Clinicopathological and Molecular Characterization of Biphasic Hyalinizing Psammomatous Renal Cell Carcinoma (BHP RCC): Further Support for the Newly Proposed Entity.; Authors - Xiao-Tong Wang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kenzo (Verified Customer) (03-28-2024)

It worked well for immunolabeling in mouse retina tissues

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse retina

FH

Juan (Verified Customer) (04-18-2019)

It works well in Western Blot experiment.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HepG2 Cell line

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