Anticorps Monoclonal anti-PAX8

PAX8 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

4H7B3

N° de cat : 60145-4-PBS

Synonymes

PAX-8, PAX 8, Paired box protein Pax-8, Paired box protein Pax 8, paired box 8

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Galerie de données de validation

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  WB analysis of SKOV-3 using 60145-4-Ig (same clone as 60145-4-PBS)

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 60145-4-Ig (PAX8 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60145-4-PBS in a different storage buffer formulation.

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  WB analysis of human thyroid gland using 60145-4-Ig (same clone as 60145-4-PBS)

  human thyroid gland tissue were subjected to SDS PAGE followed by western blot with 60145-4-Ig (PAX8 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60145-4-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 60145-4-Ig (same clone as 60145-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60145-4-Ig (PAX8 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60145-4-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 60145-4-Ig (same clone as 60145-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60145-4-Ig (PAX8 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60145-4-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 60145-4-Ig (same clone as 60145-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 60145-4-Ig (PAX8 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60145-4-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 60145-4-Ig (same clone as 60145-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 60145-4-Ig (PAX8 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60145-4-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 60145-4-Ig (same clone as 60145-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 60145-4-Ig (PAX8 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60145-4-PBS in a different storage buffer formulation.

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  IHC staining of rat kidney using 60145-4-Ig (same clone as 60145-4-PBS)

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 60145-4-Ig (PAX8 antibody) at dilution of 1:15000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60145-4-PBS in a different storage buffer formulation.

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  IHC staining of rat kidney using 60145-4-Ig (same clone as 60145-4-PBS)

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 60145-4-Ig (PAX8 antibody) at dilution of 1:15000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60145-4-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 60145-4-Ig (same clone as 60145-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60145-4-Ig (PAX8 antibody) at dilution of 1:12800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60145-4-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 60145-4-Ig (same clone as 60145-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60145-4-Ig (PAX8 antibody) at dilution of 1:12800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60145-4-PBS in a different storage buffer formulation.

• 

  IHC staining of human thyroid cancer using 60145-4-Ig (same clone as 60145-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 60145-4-Ig (PAX8 antibody) at dilution of 1:12800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60145-4-PBS in a different storage buffer formulation.

• 

  IHC staining of human thyroid cancer using 60145-4-Ig (same clone as 60145-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 60145-4-Ig (PAX8 antibody) at dilution of 1:12800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60145-4-PBS in a different storage buffer formulation.

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  IF Staining of mouse kidney using 60145-4-Ig (same clone as 60145-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using PAX8 antibody (60145-4-Ig, Clone: 4H7B3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60145-4-PBS in a different storage buffer formulation.

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  IF Staining of SKOV-3 using 60145-4-Ig (same clone as 60145-4-PBS)

  Confocal immunofluorescent analysis of (4% PFA) fixed SKOV-3 cells using 60145-4-Ig (PAX8 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). Phalloidin stains F-actin (Red). This data was developed using the same antibody clone with 60145-4-PBS in a different storage buffer formulation.

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Informations sur le produit

60145-4-PBS cible PAX8 dans les applications de WB, IHC, IF/ICC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PAX8 Protéine recombinante Ag0306
• Nom complet: paired box 8
• Masse moléculaire calculée: 48 kDa
• Poids moléculaire observé: 48 kDa
• Numéro d’acquisition GenBank: BC001060
• Symbole du gène: PAX8
• Identification du gène (NCBI): 7849
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PAX8 is a member of the paired box (PAX) family of transcription factors, typically containing a paired box domain and a paired-type homeodomain. It is expressed during organogenesis of the thyroid gland, kidney and Mullerian system. It is thought to regulate the expression of Wilms tumor suppressor (WT1) gene and mutations in PAX8 have been associated with Wilms tumor cells, thyroid and ovarian carcinomas. PAX8 is a useful marker in distinguishing ovarian carcinomas from mammary carcinomas (PMID: 18724243). PAX8 is expressed in a high percentage of ovarian serous, endometrioid, and clear cell carcinomas, but only rarely in primary ovarian mucinous adenocarcinomas.

PAX8 has 5 isoforms with calculated MW 31kd, 34kd, 41kd, 43kd and 48kd. And there is a 60kd band corresponding to the electrophoretic mobility of PAX8 (PMID:15650356).

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