Anticorps Recombinant de lapin anti-PAX8

PAX8 Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

6B14

N° de cat : 80756-1-PBS

Synonymes

PAX-8, PAX 8, Paired box protein Pax-8, Paired box protein Pax 8, paired box 8

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Galerie de données de validation

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  WB analysis using 80756-1-RR (same clone as 80756-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 80756-1-RR (PAX8 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80756-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 80756-1-RR (same clone as 80756-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 80756-1-RR (PAX8 antibody) at dilution of 1:8300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80756-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 80756-1-RR (same clone as 80756-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 80756-1-RR (PAX8 antibody) at dilution of 1:8300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80756-1-PBS in a different storage buffer formulation.

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  IHC staining of human renal cell carcinoma using 80756-1-RR (same clone as 80756-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 80756-1-RR (PAX8 antibody) at dilution of 1:8300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80756-1-PBS in a different storage buffer formulation.

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  IHC staining of human renal cell carcinoma using 80756-1-RR (same clone as 80756-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 80756-1-RR (PAX8 antibody) at dilution of 1:8300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80756-1-PBS in a different storage buffer formulation.

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  IHC staining of human thyroid cancer using 80756-1-RR (same clone as 80756-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 80756-1-RR (PAX8 antibody) at dilution of 1:8300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80756-1-PBS in a different storage buffer formulation.

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  IHC staining of human thyroid cancer using 80756-1-RR (same clone as 80756-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 80756-1-RR (PAX8 antibody) at dilution of 1:8300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80756-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 80756-1-RR (same clone as 80756-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 80756-1-RR (PAX8 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80756-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 80756-1-RR (same clone as 80756-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 80756-1-RR (PAX8 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80756-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary cancer using 80756-1-RR (same clone as 80756-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 80756-1-RR (PAX8 antibody) at dilution of 1:8000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80756-1-PBS in a different storage buffer formulation.

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  IF Staining of SKOV-3 using 80756-1-RR (same clone as 80756-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed SKOV-3 cells using PAX8 antibody (80756-1-RR, Clone: 6B14 ) at dilution of 1:1300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 80756-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 80756-1-RR

  Biolayer interferometry (BLl) kinetic assays of 80756-1-RR against Human PAX8 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

80756-1-PBS cible PAX8 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: PAX8 Protéine recombinante Ag0306
• Nom complet: paired box 8
• Masse moléculaire calculée: 48 kDa
• Poids moléculaire observé: 48 kDa, 58 kDa
• Numéro d’acquisition GenBank: BC001060
• Symbole du gène: PAX8
• Identification du gène (NCBI): 7849
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PAX8 is a member of the paired box (PAX) family of transcription factors, typically containing a paired box domain and a paired-type homeodomain. It is expressed during organogenesis of the thyroid gland, kidney and Mullerian system. It is thought to regulate the expression of Wilms tumor suppressor (WT1) gene and mutations in PAX8 have been associated with Wilms tumor cells, thyroid and ovarian carcinomas. PAX8 is a useful marker in distinguishing ovarian carcinomas from mammary carcinomas (PMID: 18724243). PAX8 is expressed in a high percentage of ovarian serous, endometrioid, and clear cell carcinomas, but only rarely in primary ovarian mucinous adenocarcinoma.

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