Anticorps Polyclonal de lapin anti-PAX8

• Phare
• Validé par KD/KO

PAX8 Polyclonal Antibody for IF/ICC, FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

IF/ICC, FC (Intra)

Conjugaison

CoraLite®594 Fluorescent Dye

Publications(1)

N° de cat : CL594-10336

Synonymes

PAX-8, paired box 8, Paired box protein Pax 8, Paired box protein Pax-8, PAX 8

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Galerie de données de validation

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  IF Staining of SKOV-3 using CL594-10336

  Immunofluorescent analysis of (4% PFA) fixed SKOV-3 cells using CoraLite®594 PAX8 antibody (CL594-10336) at dilution of 1:200.

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  FC experiment of SKOV-3 using CL594-10336

  1X10^6 SKOV-3 cells were intracellularly stained with 0.2 ug CoraLite®594 Anti-Human PAX8 (CL594-10336) (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en IF/ICC: cellules SKOV-3,
• Résultats positifs en FC (Intra): cellules SKOV-3

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 1 publications below

Informations sur le produit

CL594-10336 cible PAX8 dans les applications de IF/ICC, FC (Intra) et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PAX8 Protéine recombinante Ag0306
• Nom complet: paired box 8
• Masse moléculaire calculée: 48 kDa
• Numéro d’acquisition GenBank: BC001060
• Symbole du gène: PAX8
• Identification du gène (NCBI): 7849
• Conjugaison: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PAX8 is a member of the paired box (PAX) family of transcription factors, typically containing a paired box domain and a paired-type homeodomain. It is expressed during organogenesis of the thyroid gland, kidney and Mullerian system. It is thought to regulate the expression of Wilms tumor suppressor (WT1) gene and mutations in PAX8 have been associated with Wilms tumor cells, thyroid and ovarian carcinomas. PAX8 is a useful marker in distinguishing ovarian carcinomas from mammary carcinomas (PMID: 18724243). PAX8 is expressed in a high percentage of ovarian serous, endometrioid, and clear cell carcinomas, but only rarely in primary ovarian mucinous adenocarcinomas.

This antibody can detect all 5 isoforms of PAX8 (31 kDa, 34 kDa, 41 kDa, 43 kDa and 48 kDa). A 58-60 kDa form has also been reported (PMID:15650356, 1049952). Proteintech's 10336-1-AP antibody has cross-reactivities with the other PAX family members.

Publications

Species	Application	Title
	IF	Commun Biol; Spatial transcriptomic profiling of the human fallopian tube epithelium reveals region-specific gene expression patterns; Authors - Jared Sipes

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