Anticorps Polyclonal de lapin anti-PAX8

• Phare
• Validé par KD/KO

PAX8 Polyclonal Antibody for IF/ICC, IF-P

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

IF/ICC, IF-P

Conjugaison

CoraLite® Plus 647 Fluorescent Dye

Avis (1)

N° de cat : CL647-10336

Synonymes

PAX-8, paired box 8, Paired box protein Pax 8, Paired box protein Pax-8, PAX 8

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Galerie de données de validation

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  IF Staining of mouse kidney using CL647-10336

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using CoraLite® Plus 647 PAX8 antibody (CL647-10336) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse kidney using CL647-10336

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using CoraLite® Plus 647 PAX8 antibody (CL647-10336) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of SKOV-3 using CL647-10336

  Immunofluorescent analysis of (4% PFA) fixed SKOV-3 cells using CoraLite® Plus 647 PAX8 antibody (CL647-10336) at dilution of 1:200.

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Applications testées

• Résultats positifs en IF-P: tissu rénal de souris,
• Résultats positifs en IF/ICC: cellules SKOV-3,

Dilution recommandée

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL647-10336 cible PAX8 dans les applications de IF/ICC, IF-P et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PAX8 Protéine recombinante Ag0306
• Nom complet: paired box 8
• Masse moléculaire calculée: 48 kDa
• Poids moléculaire observé: 48 kDa, 58 kDa
• Numéro d’acquisition GenBank: BC001060
• Symbole du gène: PAX8
• Identification du gène (NCBI): 7849
• Conjugaison: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PAX8 is a member of the paired box (PAX) family of transcription factors, typically containing a paired box domain and a paired-type homeodomain. It is expressed during organogenesis of the thyroid gland, kidney and Mullerian system. It is thought to regulate the expression of Wilms tumor suppressor (WT1) gene and mutations in PAX8 have been associated with Wilms tumor cells, thyroid and ovarian carcinomas. PAX8 is a useful marker in distinguishing ovarian carcinomas from mammary carcinomas (PMID: 18724243). PAX8 is expressed in a high percentage of ovarian serous, endometrioid, and clear cell carcinomas, but only rarely in primary ovarian mucinous adenocarcinomas.

This antibody can detect all 5 isoforms of PAX8 (31 kDa, 34 kDa, 41 kDa, 43 kDa and 48 kDa). A 58-60 kDa form has also been reported (PMID:15650356, 1049952). Proteintech's 10336-1-AP antibody has cross-reactivities with the other PAX family members.

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Janett (Verified Customer) (07-03-2024)

Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human fallopian tube tissue using CoraLite® Plus 647 PAX8 antibody (CL647-10336). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) in a steamer for 20min and Antibody Dilution 1:50

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:50
• Cell Tissue Type: FFPE / fallopian tube

CL Plus 647 PAX8 Antibody Immunofluorescence validation (1:50 dilution) in FFPE / fallopian tube (Cat no:CL647-10336)

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