Anticorps Monoclonal anti-PBLD

PBLD Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1G11D4

N° de cat : 68317-1-PBS

Synonymes

FLJ14767, FLJ35507, MAWBP, MAWD binding protein, MAWDBP, PBLD

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Galerie de données de validation

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  WB analysis of pig liver using 68317-1-Ig (same clone as 68317-1-PBS)

  pig liver tissue were subjected to SDS PAGE followed by western blot with 68317-1-Ig (PBLD antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68317-1-PBS in a different storage buffer formulation.

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  WB analysis of pig kidney using 68317-1-Ig (same clone as 68317-1-PBS)

  pig kidney tissue were subjected to SDS PAGE followed by western blot with 68317-1-Ig (PBLD antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68317-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 68317-1-Ig (same clone as 68317-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using PBLD antibody (68317-1-Ig, Clone: 1G11D4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68317-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68317-1-PBS cible PBLD dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc

• Réactivité: Humain, porc
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PBLD Protéine recombinante Ag23827
• Nom complet: phenazine biosynthesis-like protein domain containing
• Poids moléculaire observé: 30 kDa
• Numéro d’acquisition GenBank: BC009738
• Symbole du gène: PBLD
• Identification du gène (NCBI): 64081
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PBLD, also termed as MAWBP, was first identified from a human liver cDNA library using a yeast two-hybrid technique by Iriyama et al. As the only representative of phenazine biosynthesis-like protein family, PBLD is widely expressed in human tissues, such as brain, heart, lung, liver, pancreas, kidney, and placenta. Its expression is elevated in several diseases processes, including insulin resistance, folate deficiency, and hypotension. PBLD has been proved to be involved in gastric carcinogenesis, PBLD expression was attenuated in gastric cancer (GC) tissues, and negatively regulated the growth and invasion in gastric cancer cell-lines by inhibiting TGF-β1-induced EMT (Epithelial-Mesenchymal Transition).(PMID: 11355021, 17929853, 23687415, 26594798)

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