Anticorps Polyclonal de lapin anti-PBX3

• Phare
• Validé par KD/KO

PBX3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, IP, chIP, RIP, ELISA

Conjugaison

Non conjugué

Publications(18)

N° de cat : 12571-1-AP

Synonymes

Homeobox protein PBX3, pre B cell leukemia homeobox 3, Pre-B-cell leukemia transcription factor 3

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Galerie de données de validation

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  WB analysis of human lung using 12571-1-AP

  human lung tissue were subjected to SDS PAGE followed by western blot with 12571-1-AP (PBX3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HL-60 using 12571-1-AP

  IP result of anti-PBX3 (IP:12571-1-AP, 4ug; Detection:12571-1-AP 1:500) with HL-60 cells lysate 3040ug.

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  IHC staining of human lung cancer using 12571-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 12571-1-AP (PBX3 antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HeLa using 12571-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PBX3 antibody (12571-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: tissu pulmonaire humain
• Résultats positifs en IP: cellules HL-60
• Résultats positifs en IHC: tissu de cancer du poumon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 9 publications below
• IHC: See 3 publications below
• IF: See 3 publications below
• IP: See 1 publications below
• ChIP: See 4 publications below
• RIP: See 1 publications below

Informations sur le produit

12571-1-AP cible PBX3 dans les applications de WB, IHC, IF/ICC, IP, chIP, RIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, poulet, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PBX3 Protéine recombinante Ag3239
• Nom complet: pre-B-cell leukemia homeobox 3
• Masse moléculaire calculée: 434 aa, 47 kDa
• Poids moléculaire observé: 47-50 kDa
• Numéro d’acquisition GenBank: BC016977
• Symbole du gène: PBX3
• Identification du gène (NCBI): 5090
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PBX3 is a homeodomain-containing transcription factor of the pre-B cell leukemia (PBX) family, members of which have extensive roles in early development and some adult processes. It plays crucial roles in embryonic development, organogenesis, and cell differentiation by regulating the expression of downstream target genes. Beyond its physiological functions, PBX3 is increasingly recognized for its significant involvement in oncogenesis. It is frequently overexpressed in various malignancies, including leukemias and solid tumors, where it promotes cell proliferation, inhibits apoptosis, enhances invasion and metastasis, and contributes to chemotherapy resistance.

Publications

Species	Application	Title
human	WB	Mol Cancer; Circular RNA circCORO1C promotes laryngeal squamous cell carcinoma progression by modulating the let-7c-5p/PBX3 axis.; Authors - Yongyan Wu
human	WB	Haematologica; P2X7 promotes the progression of MLL-AF9 induced acute myeloid leukemia by upregulation of Pbx3.; Authors - Wenli Feng ; KD Validated
mouse,human	IF	Cell Mol Gastroenterol Hepatol; scRNA-Seq Reveals New Enteric Nervous System Roles for GDNF, NRTN, and TBX3.; Authors - Christina M Wright
rat	IF	Free Radic Biol Med; Systematical exploration of transcription factors in cardiomyocytes against hydrogen peroxide-induced oxidative stress by Danhong injection.; Authors - Jingjing Zhang
mouse,human	WB,IHC	Int J Biol Sci; USF1-ATRAP-PBX3 Axis Promote Breast Cancer Glycolysis and Malignant Phenotype by Activating AKT/mTOR Signaling.; Authors - Dandan Wang
rat,human	IF	Sci Rep; Screening and identification of critical transcription factors involved in the protection of cardiomyocytes against hydrogen peroxide-induced damage by Yixin-shu.; Authors - Jingjing Zhang

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