Anticorps Polyclonal de lapin anti-PBX3

• Phare
• Validé par KD/KO

PBX3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 12571-1-PBS

Synonymes

Homeobox protein PBX3, pre B cell leukemia homeobox 3, Pre-B-cell leukemia transcription factor 3

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Galerie de données de validation

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  WB analysis of human lung using 12571-1-AP (same clone as 12571-1-PBS)

  human lung tissue were subjected to SDS PAGE followed by western blot with 12571-1-AP (PBX3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12571-1-PBS in a different storage buffer formulation.

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  IP experiment of HL-60 using 12571-1-AP (same clone as 12571-1-PBS)

  IP result of anti-PBX3 (IP:12571-1-AP, 4ug; Detection:12571-1-AP 1:500) with HL-60 cells lysate 3040ug. This data was developed using the same antibody clone with 12571-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 12571-1-AP (same clone as 12571-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 12571-1-AP (PBX3 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12571-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 12571-1-AP (same clone as 12571-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PBX3 antibody (12571-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 12571-1-PBS in a different storage buffer formulation.

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Informations sur le produit

12571-1-PBS cible PBX3 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PBX3 Protéine recombinante Ag3239
• Nom complet: pre-B-cell leukemia homeobox 3
• Masse moléculaire calculée: 434 aa, 47 kDa
• Poids moléculaire observé: 47-50 kDa
• Numéro d’acquisition GenBank: BC016977
• Symbole du gène: PBX3
• Identification du gène (NCBI): 5090
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PBX3 is a homeodomain-containing transcription factor of the pre-B cell leukemia (PBX) family, members of which have extensive roles in early development and some adult processes. It plays crucial roles in embryonic development, organogenesis, and cell differentiation by regulating the expression of downstream target genes. Beyond its physiological functions, PBX3 is increasingly recognized for its significant involvement in oncogenesis. It is frequently overexpressed in various malignancies, including leukemias and solid tumors, where it promotes cell proliferation, inhibits apoptosis, enhances invasion and metastasis, and contributes to chemotherapy resistance.

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