Anticorps Polyclonal de lapin anti-Pyruvate Carboxylase

• Phare
• Validé par KD/KO

Pyruvate Carboxylase Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

33

Avis (1)

N° de cat : 16588-1-AP

Synonymes

PC, PCB, pyruvate carboxykinase, pyruvate Carboxylase, Pyruvic carboxylase

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Galerie de données de validation

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  WB analysis using 16588-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16588-1-AP (Pyruvate Carboxylase antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 16588-1-AP

  WB result of Pyruvate Carboxylase antibody (16588-1-AP; 1:80000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Pyruvate Carboxylase transfected HepG2 cells.

• 

  IP experiment of HepG2 using 16588-1-AP

  IP result of anti-Pyruvate Carboxylase (IP:16588-1-AP, 4ug; Detection:16588-1-AP 1:1000) with HepG2 cells lysate 2640 ug.

• 

  IP experiment of mouse liver using 16588-1-AP

  IP Result of anti-Pyruvate Carboxylase (IP:16588-1-AP, 4ug; Detection:16588-1-AP 1:600) with mouse liver tissue lysate 4000ug.

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  IHC staining of human liver cancer using 16588-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16588-1-AP (Pyruvate Carboxylase antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 16588-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16588-1-AP (Pyruvate Carboxylase antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 16588-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16588-1-AP (Pyruvate Carboxylase antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 16588-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16588-1-AP (Pyruvate Carboxylase antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 16588-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16588-1-AP (Pyruvate Carboxylase antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 16588-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16588-1-AP (Pyruvate Carboxylase antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 16588-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 16588-1-AP (Pyruvate Carboxylase antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). F-actin is stained using CL555-phalloidin (red) and DNA is stained by DAPI (blue).

• 

  IF Staining of HepG2 using 16588-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 16588-1-AP (Pyruvate Carboxylase antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris, cellules HepG2, tissu hépatique de rat
• Résultats positifs en IP: cellules HepG2, tissu hépatique de souris
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 26 publications below
• IHC: See 5 publications below
• IF: See 3 publications below
• IP: See 2 publications below

Informations sur le produit

16588-1-AP cible Pyruvate Carboxylase dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, bovin, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Pyruvate Carboxylase Protéine recombinante Ag8857
• Nom complet: pyruvate carboxylase
• Masse moléculaire calculée: 1178 aa, 130 kDa
• Poids moléculaire observé: 125-130 kDa
• Numéro d’acquisition GenBank: BC011617
• Symbole du gène: PC
• Identification du gène (NCBI): 5091
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PC(pyruvate carboxylase) is a member of the family of biotin-dependent carboxylases and is found widely among eukaryotic tissues and in many prokaryotic species. It catalyses the ATP-dependent carboxylation of pyruvate to form oxaloacetate which may be utilised in the synthesis of glucose, fat, some amino acids or their derivatives and several neurotransmitters. Diabetes and hyperthyroidism increase the level of expression of pyruvate carboxylase in the long term, while its activity in the short term is controlled by the intramitochondrial concentrations of acetyl-CoA and pyruvate(PMID:9597748).

Publications

Species	Application	Title
human	WB	Nat Commun; Pyruvate anaplerosis is a targetable vulnerability in persistent leukaemic stem cells; Authors - Kevin M Rattigan
mouse	WB	Nat Commun; Enhancement of anaerobic glycolysis - a role of PGC-1α4 in resistance exercise.; Authors - Jin-Ho Koh
human	IP	Nat Commun; The long noncoding RNA ADIPINT regulates human adipocyte metabolism via pyruvate carboxylase.; Authors - Alastair G Kerr
human	WB	Nat Commun; SARS-CoV-2 infection rewires host cell metabolism and is potentially susceptible to mTORC1 inhibition.; Authors - Peter J Mullen
mouse	WB	Cancer Res; A short isoform of spermatogenic enzyme GAPDHS functions as a metabolic switch and limits metastasis in melanoma.; Authors - Jennifer G Gill
human,mouse	WB,IHC,IP	Oxid Med Cell Longev; LINC00092 Modulates Oxidative Stress and Glycolysis of Breast Cancer Cells via Pyruvate Carboxylase-Mediated AKT/mTOR Pathway.; Authors - Wei Chen

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Kevin (Verified Customer) (02-10-2022)

This worked as well as other much more expensive antibodies from differenet suppliers

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: K562