Anticorps Polyclonal de lapin anti-PCBP2

• Phare
• Validé par KD/KO

PCBP2 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

10

N° de cat : 15070-1-AP

Synonymes

Alpha CP2, hnRNP E2, HNRPE2, PCBP2, poly(rC) binding protein 2

---

Galerie de données de validation

• 

  WB analysis using 15070-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15070-1-AP (PCBP2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 15070-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 15070-1-AP (PCBP2 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human testis using 15070-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 15070-1-AP (PCBP2 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human colon using 15070-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 15070-1-AP (PCBP2 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human colon using 15070-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 15070-1-AP (PCBP2 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human liver using 15070-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15070-1-AP (PCBP2 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human cervical cancer using 15070-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 15070-1-AP (PCBP2 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 15070-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15070-1-AP (PCBP2 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human cervical cancer using 15070-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 15070-1-AP (PCBP2 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 15070-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 15070-1-AP (PCBP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Applications testées

• Résultats positifs en WB: cellules HeLa, cellules Jurkat
• Résultats positifs en IHC: tissu testiculaire humain, tissu de cancer du col de l'utérus humain, tissu de côlon humain, tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 9 publications below
• IHC: See 3 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

15070-1-AP cible PCBP2 dans les applications de WB, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PCBP2 Protéine recombinante Ag7102
• Nom complet: poly(rC) binding protein 2
• Masse moléculaire calculée: 39 kDa
• Poids moléculaire observé: 43 kDa
• Numéro d’acquisition GenBank: BC001155
• Symbole du gène: PCBP2
• Identification du gène (NCBI): 5094
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Poly(rC)-binding protein 2 (PCBP2) is a single-stranded nucleic acid binding protein that binds preferentially to oligo dC, It is also named Heterogeneous nuclear ribonucleoprotein E2, binds also poly(rU). It negatively regulates cellular antiviral responses mediated by MAVS signaling. It acts as an adapter between MAVS and the E3 ubiquitin ligase ITCH, therefore triggering MAVS ubiquitinationa and degradation.(19881509) This antibody can recognize PCBP2 and PCBP1.

Publications

Species	Application	Title
human	WB,CoIP	Nat Commun; PCBP2 maintains antiviral signaling homeostasis by regulating cGAS enzymatic activity via antagonizing its condensation.; Authors - Haiyan Gu ; KD Validated
human	WB	Virol Sin; Ectopic Expression of TRIM25 Restores RIG-I Expression and IFN Production Reduced by Multiple Enteroviruses 3Cpro.; Authors - Huimin Xiao
human	IHC,WB	Mol Cancer Res; PCBP2 Posttranscriptional Modifications Induce Breast Cancer Progression via Upregulation of UFD1 and NT5E.; Authors - Xiaonan Wang ; KD Validated
human	WB,IF	Vet Microbiol; Cellular poly(C) binding protein 2 interacts with porcine epidemic diarrhea virus papain-like protease 1 and supports viral replication.; Authors - Pengfei Zhang ; KD Validated
rat	WB, IHC	Cell Mol Neurobiol; Spatiotemporal Expression of Poly(rC)-Binding Protein PCBP2 Modulates Schwann Cell Proliferation After Sciatic Nerve Injury.; Authors - Zhigang Chen
human	WB,IHC	FEBS Open Bio; Poly(rC) binding protein 2 (PCBP2) promotes the viability of human gastric cancer cells by regulating CDK2.; Authors - Changyu Chen ; KD Validated