Anticorps Monoclonal anti-PCCB

• Phare
• Validé par KD/KO

PCCB Monoclonal Antibody for WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1A9A5

N° de cat : 66501-1-PBS

Synonymes

1A9A5, EC:6.4.1.3, PCCase subunit beta, Propanoyl-CoA:carbon dioxide ligase subunit beta, Propionyl-CoA carboxylase beta chain, mitochondrial

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Galerie de données de validation

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  WB analysis using 66501-1-Ig (same clone as 66501-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66501-1-Ig (PCCB antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66501-1-PBS in a different storage buffer formulation.

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  WB analysis using 66501-1-Ig (same clone as 66501-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66501-1-Ig (PCCB antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66501-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 66501-1-Ig (same clone as 66501-1-PBS)

  WB result of PCCB antibody (66501-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PCCB transfected HepG2 cells. This data was developed using the same antibody clone with 66501-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 66501-1-Ig (same clone as 66501-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66501-1-Ig (PCCB antibody) at dilution of 1:300 (under 10x lens). This data was developed using the same antibody clone with 66501-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 66501-1-Ig (same clone as 66501-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66501-1-Ig (PCCB antibody) at dilution of 1:300 (under 40x lens). This data was developed using the same antibody clone with 66501-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse kidney using 66501-1-Ig (same clone as 66501-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using PCCB antibody (66501-1-Ig, Clone: 1A9A5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66501-1-PBS in a different storage buffer formulation.

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  IF Staining of human kidney using 66501-1-Ig (same clone as 66501-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using 66501-1-Ig (PCCB antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66501-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50335-2

  Cytometric bead array standard curve of MP50335-2, PCCB Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66501-2-PBS. Detection antibody: 66501-1-PBS. Standard:Ag1608. Range: 0.781-100 ng/mL.

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  Cytometric bead array standard curve of MP50335-3

  Cytometric bead array standard curve of MP50335-3, PCCB Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66501-1-PBS. Detection antibody: 66501-3-PBS. Standard:Ag1608. Range: 0.781-100 ng/mL.

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  Affinity and Kinetic Characterization of 66501-1-Ig

  Biolayer interferometry (BLl) kinetic assay of 66501-1-Ig against Human PCCB was performed. The affinity constant is 2.99 nM.

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Informations sur le produit

66501-1-PBS cible PCCB dans les applications de WB, IHC, IF-P, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PCCB Protéine recombinante Ag1608
• Nom complet: propionyl Coenzyme A carboxylase, beta polypeptide
• Masse moléculaire calculée: 58 kDa
• Poids moléculaire observé: 58 kDa
• Numéro d’acquisition GenBank: BC013768
• Symbole du gène: PCCB
• Identification du gène (NCBI): 5096
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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