Anticorps Polyclonal de lapin anti-Progranulin/PGRN

Progranulin/PGRN Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(3)

N° de cat : 10053-1-AP

Synonymes

granulin, Granulin-1, Granulin precursor, Glycoprotein of 88 Kda, Glycoprotein 88

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Galerie de données de validation

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  WB analysis using 10053-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10053-1-AP (Granulin antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10053-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10053-1-AP (Granulin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 10053-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10053-1-AP (Granulin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 10053-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10053-1-AP (Granulin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 10053-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10053-1-AP (Granulin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 10053-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10053-1-AP (Granulin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 10053-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10053-1-AP (Granulin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules HEK-293, cellules MCF-7
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 1 publications below
• IF: See 2 publications below

Informations sur le produit

10053-1-AP cible Progranulin/PGRN dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Progranulin/PGRN Protéine recombinante Ag0010
• Nom complet: granulin
• Masse moléculaire calculée: 64 kDa
• Poids moléculaire observé: 64 kDa
• Numéro d’acquisition GenBank: BC010577
• Symbole du gène: Granulin
• Identification du gène (NCBI): 2896
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GRN, also known as PGRN or PCDGF, is a cysteine-rich protein of 68.5 kDa that is typically secreted into a highly glycosylated 88 kDa form. PGRN is a unique growth factor that plays an important role in cutaneous wound healing. It has an anti-inflammatory effect and promotes cell proliferation. When PCDGF is degraded to several 6-25 kDa fragments, called granulins (GRNs) by neutrophil proteases, a pro-inflammatory reaction occurs. PGRN is widely expressed, particularly in epithelial cells, immune cells, neurons, and chondrocytes. High levels of PGRN expression have been reported in human cancers, and its expression is closely correlated with the development and metastasis of several cancers. The recent discovery that mutations in the gene encoding for pro-granulin (GRN) cause frontotemporal lobar degeneration (FTLD), and other neurodegenerative diseases leading to dementia, has brought renewed interest in progranulin and its functions in the central nervous system. Several in vitro studies have revealed that PGRN is classified into two isoforms according to its glycosylation status, the glycosylated immature isoform (58-68 kDa) and the fully glycosylated mature secretory isoform (∼88 kDa) (PMID: 25838514).

Publications

Species	Application	Title
human	IHC	Chin Med J (Engl); Expression of PC cell-derived growth factor and vascular endothelial growth factor in esophageal squamous cell carcinoma and their clinicopathologic significance.; Authors - Chen Xiang-yu XY
	IF	Cell Prolif; m6 A promotes planarian regeneration; Authors - Guanshen Cui
human	IF	PeerJ; Integrated multiomics analysis highlights the immunosuppressive role of granulin precursor positive macrophages in hepatocellular carcinoma; Authors - Jun Li

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