Anticorps Polyclonal de lapin anti-Progranulin/PGRN

• Phare
• Validé par KD/KO

Progranulin/PGRN Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

12

Avis (1)

N° de cat : 18410-1-AP

Synonymes

acrogranin, GEP, GP88, granulin, Granulin A Granulin 5, Granulin B Granulin 4, Granulin C Granulin 6, Granulin D Granulin 7, Granulin E, Granulin F Granulin 3, Granulin G Granulin 2, Granulins, GRN, PCDGF, PEPI, PGRN, Proepithelin

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Galerie de données de validation

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  WB analysis using 18410-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18410-1-AP (Granulin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 18410-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 18410-1-AP (Granulin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 18410-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 18410-1-AP (Granulin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 18410-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 18410-1-AP (Granulin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 18410-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 18410-1-AP (Granulin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-937 using 18410-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 18410-1-AP (Granulin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 18410-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 18410-1-AP (Granulin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human saliva using 18410-1-AP

  human saliva were subjected to SDS PAGE followed by western blot with 18410-1-AP (Granulin antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 18410-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 18410-1-AP (Granulin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 18410-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18410-1-AP (Granulin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of THP-1 using 18410-1-AP

  THP-1 cells were subjected to SDS PAGE followed by western blot with 18410-1-AP (Granulin antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas cancer using 18410-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 18410-1-AP (Granulin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 18410-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 18410-1-AP (Granulin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 18410-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18410-1-AP (Granulin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 18410-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18410-1-AP (Granulin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 18410-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 18410-1-AP (Granulin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 18410-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 18410-1-AP (Granulin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 18410-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 18410-1-AP (Granulin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 18410-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 18410-1-AP (Granulin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 18410-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 18410-1-AP (Granulin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 18410-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 18410-1-AP (Granulin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules A431, cellules HEK-293, cellules HeLa, cellules PC-3, cellules RAW 264.7, cellules THP-1, cellules U-937, salive humain, salive humaine, tissu cardiaque de souris, tissu cérébral de rat, tissu pulmonaire de souris
• Résultats positifs en IHC: tissu de cancer du pancréas humain, tissu de cancer de l'estomac humain, tissu de cancer du poumon humain, tissu de tumeur ovarienne humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 6 publications below
• IF: See 2 publications below

Informations sur le produit

18410-1-AP cible Progranulin/PGRN dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Progranulin/PGRN Protéine recombinante Ag13178
• Nom complet: granulin
• Masse moléculaire calculée: 64 kDa
• Poids moléculaire observé: 60-70 kDa
• Numéro d’acquisition GenBank: BC000324
• Symbole du gène: GRN
• Identification du gène (NCBI): 2896
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GRN, also known as PGRN or PCDGF, is a cysteine-rich protein of 68.5 kDa that is typically secreted into a highly glycosylated 88 kDa form. PGRN is a unique growth factor that plays an important role in cutaneous wound healing. It has an anti-inflammatory effect and promotes cell proliferation. When PCDGF is degraded to several 6-25 kDa fragments, called granulins (GRNs) by neutrophil proteases, a pro-inflammatory reaction occurs. PGRN is widely expressed, particularly in epithelial cells, immune cells, neurons, and chondrocytes. High levels of PGRN expression have been reported in human cancers, and its expression is closely correlated with the development and metastasis of several cancers. The recent discovery that mutations in the gene encoding for pro-granulin (GRN) cause frontotemporal lobar degeneration (FTLD), and other neurodegenerative diseases leading to dementia, has brought renewed interest in progranulin and its functions in the central nervous system.

Publications

Species	Application	Title
rat	WB	Transl Psychiatry; Progranulin improves neural development via the PI3K/Akt/GSK-3β pathway in the cerebellum of a VPA-induced rat model of ASD.; Authors - Lili Wang
human	IHC	J Proteome Res; Mining the gastric cancer secretome: identification of GRN as a potential diagnostic marker for early gastric cancer.; Authors - Loei Hendrick H
human	IF	J Mol Med (Berl); Progranulin alleviates podocyte injury via regulating CAMKK/AMPK-mediated autophagy under diabetic conditions.; Authors - Di Zhou
human	WB	Front Oncol; GRN is a prognostic biomarker and correlated with immune infiltration in glioma: A study based on TCGA data; Authors - Su-Mei Xu
rat	WB	Neuropharmacology; Abnormal spatiotemporal expression pattern of progranulin and neurodevelopment impairment in VPA-induced ASD rat model.; Authors - Junying Lan
rat	WB,IHC,IF	Int J Mol Sci; Involvement of Progranulin and Granulin Expression in Inflammatory Responses after Cerebral Ischemia.; Authors - Ichiro Horinokita

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Sharan (Verified Customer) (06-24-2020)

The antibody detects the GRN protein but there is also a lot of cross-reactivity with non-specific antigens in HeLa lysate

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HeLa cells

Progranulin/PGRN Antibody Western Blot, validation (1:1000 dilution) in HeLa cells (Cat no:18410-1-AP)