Anticorps Polyclonal de lapin anti-PCDH10

• Phare
• Validé par KD/KO

PCDH10 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(5)

Avis (2)

N° de cat : 21859-1-AP

Synonymes

KIAA1400, Protocadherin-10

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Galerie de données de validation

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  WB analysis of PC-3 using 21859-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 21859-1-AP (PCDH10 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis using 21859-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21859-1-AP (PCDH10 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 21859-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21859-1-AP (PCDH10 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 21859-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21859-1-AP (PCDH10 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of PC-3 using 21859-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed PC-3 cells using 21859-1-AP (PCDH10 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: SK-N-SH cells, cellules PC-3
• Résultats positifs en IHC: tissu cérébral de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules PC-3

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 1 publications below

Informations sur le produit

21859-1-AP cible PCDH10 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PCDH10 Protéine recombinante Ag16494
• Nom complet: protocadherin 10
• Masse moléculaire calculée: 1040 aa, 113 kDa
• Poids moléculaire observé: 113-130 kDa
• Numéro d’acquisition GenBank: BC111560
• Symbole du gène: PCDH10
• Identification du gène (NCBI): 57575
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Theranostics; A Compound AC1Q3QWB Selectively Disrupts HOTAIR-Mediated Recruitment of PRC2 and Enhances Cancer Therapy of DZNep.; Authors - Yansheng Li
human	WB	Clin Transl Med; Hsa_circ_0001666 suppresses the progression of colorectal cancer through the miR-576-5p/PCDH10 axis.; Authors - Jiahui Zhou
human	WB	Int J Biol Sci; FOXM1-induced miR-552 expression contributes to pancreatic cancer progression by targeting multiple tumor suppressor genes.; Authors - Xiao Wang ; KD Validated
human	IF	Nature; Shifts in receptors during submergence of an encephalitic arbovirus; Authors - Wanyu Li
		Cell; Structural basis for plasticity in receptor engagement by an encephalitic alphavirus; Authors - Saravanan Raju

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Sampath (Verified Customer) (03-11-2020)

Pcdh10 antibody worked very well for me. I saw the bands even after 5 sec of exposure at dilution 1:1000 o/n incubation.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Brain tissue

PCDH10 Antibody Western Blot, validation (1:1000 dilution) in Brain tissue (Cat no:21859-1-AP)

FH

Aurelie (Verified Customer) (06-13-2019)

In tissue, there is some background but the antibody give a correct signal. I also try in human primary fibroblasts, the background is lower and the band at 130kd observed here , is not present.- dilution 1/1500 should be enough

• Applications: Western Blot,
• Primary Antibody Dilution: 1/800
• Cell Tissue Type: Mouse Cortex

PCDH10 Antibody Western Blot, validation (1/800 dilution) in Mouse Cortex (Cat no:21859-1-AP)

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