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Anticorps Polyclonal de lapin anti-PCDH15

PCDH15 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 20719-1-PBS

Synonymes

Galerie de données de validation

Filter:
  • Western Blot (WB) analysis of various lysates using PCDH15 Polyclonal antibody (20719-1-AP)

    WB analysis using 20719-1-AP (same clone as 20719-1-PBS)

    mouse brain tissue lysates were subjected to SDS PAGE followed by western blot with 20719-1-AP (PCDH15 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20719-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PCDH15 Polyclonal antibody (20719-1-AP)

    WB analysis using 20719-1-AP (same clone as 20719-1-PBS)

    mouse liver tissue lysates were subjected to SDS PAGE followed by western blot with 20719-1-AP (PCDH15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20719-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using PCDH15 Polyclonal antibody (20719-1-AP)

    IF Staining of HEK-293 using 20719-1-AP (same clone as 20719-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using PCDH15 antibody (20719-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 20719-1-PBS in a different storage buffer formulation.

Informations sur le produit

20719-1-PBS cible PCDH15 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

Réactivité Humain, rat, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène Peptide
Nom complet protocadherin 15
Masse moléculaire calculée 216 kDa
Poids moléculaire observé120-160 kDa, 50 kDa
Numéro d’acquisition GenBankNM_033056
Symbole du gène PCDH15
Identification du gène (NCBI) 65217
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS only
Conditions de stockageStore at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Protocadherin-15 (PCDH15) belongs to the cadherin superfamily that mediates calcium-dependent cell-cell adhesion and plays an important role in the maintenance of normal tissue architecture. A homozygous variant in PCDH15 results in Usher syndrome type 1F (USH1F) which leads to congenital hearing loss and syndromicretinitis pigmentosa (PMID: 38014037). Multiple isoforms of PCDH15 have been described, including 216-250 kDa (full-length), 150-180 kDa, 100-120 kDa, 50-65 kDa, and 25-40 kDa (PMID: 22363448; 15928608; 14570705; 23035094; 28883094).

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