Anticorps Polyclonal de lapin anti-PCDH9

PCDH9 Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 25090-1-AP

Synonymes

PCDH9, protocadherin 9

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Galerie de données de validation

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  WB analysis of mouse brain using 25090-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25090-1-AP (PCDH9 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 25090-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 25090-1-AP (PCDH9 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 25090-1-AP

  IP Result of anti-PCDH9 (IP:25090-1-AP, 4ug; Detection:25090-1-AP 1:1000) with mouse brain tissue lysate 5000ug.

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  IHC staining of human gliomas using 25090-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 25090-1-AP (PCDH9 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 25090-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 25090-1-AP (PCDH9 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 25090-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25090-1-AP (PCDH9 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 25090-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25090-1-AP (PCDH9 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu de gliome humain, tissu cérébral de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

25090-1-AP cible PCDH9 dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PCDH9 Protéine recombinante Ag18696
• Nom complet: protocadherin 9
• Masse moléculaire calculée: 1237 aa, 136 kDa
• Poids moléculaire observé: 150-180 kDa
• Numéro d’acquisition GenBank: BC136626
• Symbole du gène: PCDH9
• Identification du gène (NCBI): 5101
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Protocadherin-9 (PCDH9) is a member of the protocadherin family that belongs to the cadherin superfamily. Protocadherins are calcium-dependent adhesion proteins and have been implicated in neural cell-cell interactions. They are abundantly expressed in the central nervous system during embryonic development and in adulthood (PMID: 24214103). PCDH9 is a single-pass type I membrane protein that contains seven cadherin motifs in its extracellular domain. It might be involved in the formation of specific neural circuits during neural development (PMID: 22982106).

What is the molecular weight of PCDH9?

The calculated molecular weight of PCDH9 is 136 kDa.

What are the isoforms of PCDH9?

Alternatively spliced transcript variants encoding distinct isoforms have been identified for the PCDH9 gene; a short transcript, a standard transcript, a transcript with additional exon 2, and another transcript with variant exon 3 (PMID: 15095963).

What is PCDH9's involvement in neural development?

The family of protocadherins is essential for neural development as mutations in these proteins give rise to neurodevelopmental disorders such as schizophrenia and epilepsy. However, the exact mechanisms of loss of function and the role of PCDH9 remain to be fully characterized (PMID: 22982106).

Publications

Species	Application	Title
mouse	WB	Neuron; Longitudinal single-cell transcriptional dynamics throughout neurodegeneration in SCA1; Authors - Leon Tejwani
human	WB	Onco Targets Ther; MiR-200a-3p promoted the malignant behaviors of ovarian cancer cells through regulating PCDH9.; Authors - Can Shi