Anticorps Polyclonal de lapin anti-PCDHA2

PCDHA2 Polyclonal Antibody for WB, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 10127-2-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 10127-2-AP (same clone as 10127-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10127-2-AP (PCDHA2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10127-2-PBS in a different storage buffer formulation.

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  WB analysis of Raji using 10127-2-AP (same clone as 10127-2-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 10127-2-AP (PCDHA2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10127-2-PBS in a different storage buffer formulation.

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  WB analysis of human heart using 10127-2-AP (same clone as 10127-2-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 10127-2-AP (PCDHA2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10127-2-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 10127-2-AP (same clone as 10127-2-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 10127-2-AP (PCDHA2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10127-2-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 10127-2-AP (same clone as 10127-2-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 10127-2-AP (PCDHA2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10127-2-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 10127-2-AP (same clone as 10127-2-PBS)

  IP result of anti-PCDHA2 (IP:10127-2-AP, 3ug; Detection:10127-2-AP 1:300) with HeLa cells lysate 4650ug. This data was developed using the same antibody clone with 10127-2-PBS in a different storage buffer formulation.

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Informations sur le produit

10127-2-PBS cible PCDHA2 dans les applications de WB, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PCDHA2 Protéine recombinante Ag0173
• Nom complet: protocadherin alpha 2
• Masse moléculaire calculée: 102 kDa
• Poids moléculaire observé: 102 kDa, 80 kDa
• Numéro d’acquisition GenBank: BC003126
• Symbole du gène: PCDHA2
• Identification du gène (NCBI): 56146
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Protocadherins, which constitute the largest subgroup within the cadherin superfamily, are predominantly expressed in the nervous system and are probably involved in the regulation of neuronal recognition and connectivity (PMID: 17936607; 12231349; 17133224). The protocadherin subfamily can be further subdivided into three groups: the clustered protocadherins, comprising α-, β- and γ-protocadherins; δ-protocadherins; and others, many of which are solitary (PMID: 17133224). PCDHA2 (protocadherin alpha 2) is a single-pass type I membrane protein containing 6 cadherin domains. It may be involved in the establishment and maintenance of specific neuronal connections in the brain. Three isoforms produced by alternative splicing have been described (UniProt).

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