Anticorps Polyclonal de lapin anti-PCF11

• Phare
• Validé par KD/KO

PCF11 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 23540-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of K-562 using 23540-1-AP (same clone as 23540-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 23540-1-AP (PCF11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23540-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 23540-1-AP (same clone as 23540-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 23540-1-AP (PCF11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23540-1-PBS in a different storage buffer formulation.

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  IP experiment of K-562 using 23540-1-AP (same clone as 23540-1-PBS)

  IP result of anti-PCF11 (IP:23540-1-AP, 4ug; Detection:23540-1-AP 1:1000) with K-562 cells lysate 2400 ug. This data was developed using the same antibody clone with 23540-1-PBS in a different storage buffer formulation.

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  IHC staining of human intrahepatic cholangiocarcinoma using 23540-1-AP (same clone as 23540-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 23540-1-AP (PCF11 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23540-1-PBS in a different storage buffer formulation.

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Informations sur le produit

23540-1-PBS cible PCF11 dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PCF11 Protéine recombinante Ag20263
• Nom complet: PCF11, cleavage and polyadenylation factor subunit, homolog (S. cerevisiae)
• Masse moléculaire calculée: 1555 aa, 173 kDa
• Poids moléculaire observé: 173 kDa
• Numéro d’acquisition GenBank: BC146778
• Symbole du gène: PCF11
• Identification du gène (NCBI): 51585
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purifié par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

In Saccharomyces cerevisiae, the cleavage/polyadenylation factor Pcf11 is a crucial regulatory factor required for recruiting polyadenylation machinery to elongating RNA polymerase II (RNAPII), and is necessary for correct transcriptional termination. Pcf11 (PCF11, cleavage and polyadenylation factor subunit, homolog (S. cerevisiae)), is a 1,555 amino acid nuclear protein that is a component of pre-mRNA cleavage complex II. It is suggested that Pcf11 is capable of promoting the dissociation of Pol II elongation complexes from DNA. Pcf11 contains a CTD-interaction domain that binds in a phospho-dependent manner to the heptad repeats within the RNA polymerase II CTD. The gene encoding Pcf11 is located on human chromosoem 11, which houses over 1,400 genes and comprises nearly 4% of the human genome. Jervell and Lange-Nielsen syndrome, Jacobsen syndrome, Niemann-Pick disease, hereditary angioedema and Smith-Lemli-Opitz syndrome are associated with defects in genes that maps to chromosome 11. This antibody is specific to the 173kd human PCF11 protein.

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