Anticorps Polyclonal de lapin anti-PCIF1

PCIF1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 16082-1-PBS

Synonymes

C20orf67, CAPAM, Cap-specific adenosine methyltransferase, EC:2.1.1.62, hCAPAM

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Galerie de données de validation

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  WB analysis using 16082-1-AP (same clone as 16082-1-PBS)

  Ramos cells were subjected to SDS PAGE followed by western blot with 16082-1-AP (PCIF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16082-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 16082-1-AP (same clone as 16082-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16082-1-AP (PCIF1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16082-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 16082-1-AP (same clone as 16082-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16082-1-AP (PCIF1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16082-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293T using 16082-1-AP (same clone as 16082-1-PBS)

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 16082-1-AP (PCIF1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16082-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 16082-1-AP (same clone as 16082-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16082-1-AP (PCIF1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16082-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 16082-1-AP (same clone as 16082-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16082-1-AP (PCIF1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16082-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 16082-1-AP (same clone as 16082-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using PCIF1 antibody (16082-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-Phalloidin (green). This data was developed using the same antibody clone with 16082-1-PBS in a different storage buffer formulation.

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  IF Staining of Caco-2 using 16082-1-AP (same clone as 16082-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using PCIF1 antibody (16082-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 16082-1-PBS in a different storage buffer formulation.

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Informations sur le produit

16082-1-PBS cible PCIF1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PCIF1 Protéine recombinante Ag9080
• Nom complet: PDX1 C-terminal inhibiting factor 1
• Masse moléculaire calculée: 704 aa, 81 kDa
• Poids moléculaire observé: 81 kDa
• Numéro d’acquisition GenBank: BC013365
• Symbole du gène: PCIF1
• Identification du gène (NCBI): 63935
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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