Anticorps Polyclonal de lapin anti-PCK2

PCK2 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

20

Avis (1)

N° de cat : 14892-1-AP

Synonymes

PCK2, PEPCK, PEPCK M, PEPCK2

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Galerie de données de validation

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  WB analysis of mouse brain using 14892-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14892-1-AP (PCK2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 14892-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 14892-1-AP (PCK2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 14892-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 14892-1-AP (PCK2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 14892-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14892-1-AP (PCK2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 14892-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14892-1-AP (PCK2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 14892-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14892-1-AP (PCK2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 14892-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14892-1-AP (PCK2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 14892-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14892-1-AP (PCK2 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver cancer using 14892-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14892-1-AP (PCK2 antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of MCF-7 using 14892-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 14892-1-AP (PCK2 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 14892-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 14892-1-AP (PCK2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat, tissu hépatique de souris
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cancer du foie humain, tissu rénal de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules MCF-7, cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:100-1:1000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 15 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Informations sur le produit

14892-1-AP cible PCK2 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PCK2 Protéine recombinante Ag6681
• Nom complet: phosphoenolpyruvate carboxykinase 2 (mitochondrial)
• Masse moléculaire calculée: 71 kDa
• Poids moléculaire observé: 66-71 kDa
• Numéro d’acquisition GenBank: BC001454
• Symbole du gène: PCK2
• Identification du gène (NCBI): 5106
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PCK2(phosphoenolpyruvate carboxykinase [GTP], mitochondrial) is also named as PEPCK2, PEPCK-M and belongs to the phosphoenolpyruvate carboxykinase [GTP] family. It catalyses the irreversible conversion of oxaloacetate to phosphoenolpyruvate, an important initial step in hepatic gluconeogenesis.Defects in PCK2 are the cause of mitochondrial phosphoenolpyruvate carboxykinase deficiency (M-PEPCKD). It has 2 isoforms produced by alternative splicing with the molecular weight of 71 kDa and 48 kDa.

Publications

Species	Application	Title
human	WB	Sci Rep; TCF7L2 involvement in estradiol- and progesterone-modulated islet and hepatic glucose homeostasis.; Authors - Fengqin Dong
mouse	WB	Mol Nutr Food Res; Douchi Peptides Vy and Sfllr Improve Glucose Homeostasis and Gut Dysbacteriosis In High-Fat Diet-Induced Insulin Resistant Mice; Authors - Songfeng Yu
mouse	WB	J Nat Prod; Effect of 1-Deoxynojirimycin Isolated from Mulberry Leaves on Glucose Metabolism and Gut Microbiota in a Streptozotocin-Induced Diabetic Mouse Model; Authors - Teng-Gen Hu
rat	IF	Mol Neurobiol; Phosphoenolpyruvate Carboxykinase (PCK) in the Brain Gluconeogenic Pathway Contributes to Oxidative and Lactic Injury After Stroke.; Authors - Xiaokun Geng
rat	WB	Front Pharmacol; Bicyclol Regulates Hepatic Gluconeogenesis in Rats with Type 2 Diabetes and Non-alcoholic Fatty Liver Disease by Inhibiting Inflammation.; Authors - Hongxue Li
mouse	WB	Food Funct; Polysaccharides from Armillariella tabescens mycelia ameliorate insulin resistance in type 2 diabetic mice.; Authors - Rui Yang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Kristian (Verified Customer) (06-07-2021)

PCK2 (red) is detected in proximale tubules where HIF1A-signaling is activated.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Transgenic mouse kidney (TRACK)

PCK2 Antibody Immunofluorescence validation (1:200 dilution) in Transgenic mouse kidney (TRACK) (Cat no:14892-1-AP)