Anticorps Polyclonal de lapin anti-PCK1/2

PCK1/2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Conjugaison

Non conjugué

Publications(35)

Avis (1)

N° de cat : 14892-1-AP

Synonymes

PCK2, Phosphoenolpyruvate carboxykinase 2, mitochondrial, PEPCK-M, PEPCK2, mtPCK2

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Galerie de données de validation

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  WB analysis of mouse liver using 14892-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 14892-1-AP (PCK1/2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using 14892-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14892-1-AP (PCK1/2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 14892-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14892-1-AP (PCK1/2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 14892-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14892-1-AP (PCK1/2 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver cancer using 14892-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14892-1-AP (PCK1/2 antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of MCF-7 using 14892-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 14892-1-AP (PCK1/2 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat, tissu hépatique de souris
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cancer du foie humain, tissu rénal de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:100-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 28 publications below
• IHC: See 5 publications below
• IF: See 3 publications below
• CoIP: See 1 publications below

Informations sur le produit

14892-1-AP cible PCK1/2 dans les applications de WB, IHC, IF/ICC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PCK1/2 Protéine recombinante Ag6681
• Nom complet: phosphoenolpyruvate carboxykinase 2 (mitochondrial)
• Masse moléculaire calculée: 71 kDa
• Poids moléculaire observé: 66-71 kDa
• Numéro d’acquisition GenBank: BC001454
• Symbole du gène: PCK2
• Identification du gène (NCBI): 5106
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PCK2(phosphoenolpyruvate carboxykinase [GTP], mitochondrial) is also named as PEPCK2, PEPCK-M and belongs to the phosphoenolpyruvate carboxykinase [GTP] family. It catalyses the irreversible conversion of oxaloacetate to phosphoenolpyruvate, an important initial step in hepatic gluconeogenesis.Defects in PCK2 are the cause of mitochondrial phosphoenolpyruvate carboxykinase deficiency (M-PEPCKD). It has 2 isoforms produced by alternative splicing with the molecular weight of 71 kDa and 48 kDa. This antibody has weak cross-reactivity with PCK1 protein due to high sequence homology between PCK1 and PCK2.

Publications

Species	Application	Title
human	WB	Sci Rep; TCF7L2 involvement in estradiol- and progesterone-modulated islet and hepatic glucose homeostasis.; Authors - Fengqin Dong
mouse	WB	Mol Nutr Food Res; Douchi Peptides Vy and Sfllr Improve Glucose Homeostasis and Gut Dysbacteriosis In High-Fat Diet-Induced Insulin Resistant Mice; Authors - Songfeng Yu
mouse	WB	J Nat Prod; Effect of 1-Deoxynojirimycin Isolated from Mulberry Leaves on Glucose Metabolism and Gut Microbiota in a Streptozotocin-Induced Diabetic Mouse Model; Authors - Teng-Gen Hu
		J Biol Chem; Patient-derived organotypic tissue cultures as a platform to evaluate metabolic reprogramming in breast cancer patients; Authors - Teresa W -M Fan
rat	IF	Mol Neurobiol; Phosphoenolpyruvate Carboxykinase (PCK) in the Brain Gluconeogenic Pathway Contributes to Oxidative and Lactic Injury After Stroke.; Authors - Xiaokun Geng
rat	WB	Front Pharmacol; Bicyclol Regulates Hepatic Gluconeogenesis in Rats with Type 2 Diabetes and Non-alcoholic Fatty Liver Disease by Inhibiting Inflammation.; Authors - Hongxue Li

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kristian (Verified Customer) (06-07-2021)

PCK2 (red) is detected in proximale tubules where HIF1A-signaling is activated.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Transgenic mouse kidney (TRACK)

PCK1/2 Antibody Immunofluorescence validation (1:200 dilution) in Transgenic mouse kidney (TRACK) (Cat no:14892-1-AP)

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