Anticorps Polyclonal de lapin anti-PCK1/2

PCK1/2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

N° de cat : 14892-1-PBS

Synonymes

PCK2, Phosphoenolpyruvate carboxykinase 2, mitochondrial, PEPCK-M, PEPCK2, mtPCK2

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Galerie de données de validation

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  WB analysis of mouse brain using 14892-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14892-1-AP (PCK2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 14892-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 14892-1-AP (PCK2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 14892-1-AP (same clone as 14892-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 14892-1-AP (PCK1/2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14892-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 14892-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14892-1-AP (PCK2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 14892-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14892-1-AP (PCK2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 14892-1-AP (same clone as 14892-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14892-1-AP (PCK1/2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14892-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 14892-1-AP (same clone as 14892-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14892-1-AP (PCK1/2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14892-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 14892-1-AP (same clone as 14892-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14892-1-AP (PCK1/2 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 14892-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 14892-1-AP (same clone as 14892-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14892-1-AP (PCK1/2 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 14892-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 14892-1-AP (same clone as 14892-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 14892-1-AP (PCK1/2 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14892-1-PBS in a different storage buffer formulation.

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Informations sur le produit

14892-1-PBS cible PCK1/2 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PCK1/2 Protéine recombinante Ag6681
• Nom complet: phosphoenolpyruvate carboxykinase 2 (mitochondrial)
• Masse moléculaire calculée: 71 kDa
• Poids moléculaire observé: 66-71 kDa
• Numéro d’acquisition GenBank: BC001454
• Symbole du gène: PCK2
• Identification du gène (NCBI): 5106
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PCK2(phosphoenolpyruvate carboxykinase [GTP], mitochondrial) is also named as PEPCK2, PEPCK-M and belongs to the phosphoenolpyruvate carboxykinase [GTP] family. It catalyses the irreversible conversion of oxaloacetate to phosphoenolpyruvate, an important initial step in hepatic gluconeogenesis.Defects in PCK2 are the cause of mitochondrial phosphoenolpyruvate carboxykinase deficiency (M-PEPCKD). It has 2 isoforms produced by alternative splicing with the molecular weight of 71 kDa and 48 kDa. This antibody has weak cross-reactivity with PCK1 protein due to high sequence homology between PCK1 and PCK2.

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