Anticorps Monoclonal anti-PCK2

PCK2 Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, Lapin, porc, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

CloneNo.

1B3F1

N° de cat : 67676-1-PBS

Synonymes

1B3F1, EC:4.1.1.32, mtPCK2, PEPCK2, PEPCK-M

---

Galerie de données de validation

• 

  WB analysis using 67676-1-Ig (same clone as 67676-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67676-1-Ig (PCK2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67676-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat liver using 67676-1-Ig (same clone as 67676-1-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 67676-1-Ig (PCK2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67676-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig liver using 67676-1-Ig (same clone as 67676-1-PBS)

  pig liver tissue were subjected to SDS PAGE followed by western blot with 67676-1-Ig (PCK2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67676-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rabbit liver using 67676-1-Ig (same clone as 67676-1-PBS)

  rabbit liver tissue were subjected to SDS PAGE followed by western blot with 67676-1-Ig (PCK2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67676-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HepG2 using 67676-1-Ig (same clone as 67676-1-PBS)

  IP result of anti-PCK2 (IP:67676-1-Ig, 4ug; Detection:67676-1-Ig 1:2000) with HepG2 cells lysate 1640 ug. This data was developed using the same antibody clone with 67676-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 67676-1-Ig (same clone as 67676-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67676-1-Ig (PCK2 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67676-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 67676-1-Ig (same clone as 67676-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67676-1-Ig (PCK2 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67676-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 67676-1-Ig (same clone as 67676-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67676-1-Ig (PCK2 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67676-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 67676-1-Ig (same clone as 67676-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67676-1-Ig (PCK2 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67676-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 67676-1-Ig (same clone as 67676-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using PCK2 antibody (67676-1-Ig, Clone: 1B3F1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 67676-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

67676-1-PBS cible PCK2 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, rat, souris

• Réactivité: Humain, Lapin, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PCK2 Protéine recombinante Ag7021
• Nom complet: phosphoenolpyruvate carboxykinase 2 (mitochondrial)
• Masse moléculaire calculée: 71 kDa
• Poids moléculaire observé: 68 kDa
• Numéro d’acquisition GenBank: BC001454
• Symbole du gène: PCK2
• Identification du gène (NCBI): 5106
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PCK2(phosphoenolpyruvate carboxykinase [GTP], mitochondrial) is also named as PEPCK2, PEPCK-M and belongs to the phosphoenolpyruvate carboxykinase [GTP] family. It catalyses the irreversible conversion of oxaloacetate to phosphoenolpyruvate, an important initial step in hepatic gluconeogenesis. Defects in PCK2 are the cause of mitochondrial phosphoenolpyruvate carboxykinase deficiency (M-PEPCKD). It has 2 isoforms produced by alternative splicing with the molecular weight of 71 kDa and 48 kDa.

{{ptg:RelatedPrimaryAntibodies}}