Anticorps Polyclonal de lapin anti-PCM1

• Phare
• Validé par KD/KO

PCM1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 19856-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 19856-1-AP (same clone as 19856-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 19856-1-AP (PCM1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19856-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 19856-1-AP (same clone as 19856-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 19856-1-AP (PCM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19856-1-PBS in a different storage buffer formulation.

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  WB analysis of HT-1080 using 19856-1-AP (same clone as 19856-1-PBS)

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 19856-1-AP (PCM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19856-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa cells using 19856-1-AP (same clone as 19856-1-PBS)

  IP result of anti-PCM1 (19856-1-AP for IP and Detection) with HeLa cells. This data was developed using the same antibody clone with 19856-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 19856-1-AP (same clone as 19856-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 19856-1-AP (PCM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19856-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 19856-1-AP (same clone as 19856-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 19856-1-AP (PCM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19856-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 19856-1-AP (same clone as 19856-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 19856-1-AP (PCM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19856-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 19856-1-AP (same clone as 19856-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 19856-1-AP (PCM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19856-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 19856-1-AP (same clone as 19856-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 19856-1-AP (PCM1 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 19856-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 19856-1-AP (same clone as 19856-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PCM1 antibody (19856-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Gamma Tubulin antibody (66320-1-Ig, Clone: 3F9H8, red). This data was developed using the same antibody clone with 19856-1-PBS in a different storage buffer formulation.

• 

  IF Staining of NIH/3T3 using 19856-1-AP (same clone as 19856-1-PBS)

  Immunofluorescent analysis of (-20℃Methanol) fixed NIH/3T3 cells using 19856-1-AP (PCM1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 19856-1-PBS in a different storage buffer formulation.

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Informations sur le produit

19856-1-PBS cible PCM1 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PCM1 Protéine recombinante Ag13929
• Nom complet: pericentriolar material 1
• Masse moléculaire calculée: 2024 aa, 228 kDa
• Poids moléculaire observé: 260-280 kDa
• Numéro d’acquisition GenBank: BC000453
• Symbole du gène: PCM1
• Identification du gène (NCBI): 5108
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Pericentriolar material 1 (PCM1) is a component of centriolar satellites, which are electron dense granules scattered around centrosomes (PMID: 10579718). PCM1 is required for centrosome assembly and function. It acts as a scaffold to target several proteins to the centrosome in a dynein motor-dependent manner and regulate microtubular dynamics (PMID:18762586; 12403812). It is also involved in the biogenesis of cilia (PMID: 18772192). Chromosomal aberrations involving PCM1 gene are associated with papillary thyroid carcinomas and a variety of hematological malignancies, including atypical chronic myeloid leukemia and T-cell lymphoma. (PMID: 10980597; 16091753; 15805263; 16424865).

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