Anticorps Recombinant de lapin anti-PCM1

PCM1 Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

242199A9

N° de cat : 84979-1-RR

Synonymes

pericentriolar material 1, PCM-1, PCM 1, hPCM-1, hPCM 1

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Galerie de données de validation

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  WB analysis using 84979-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84979-1-RR (PCM1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 84979-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84979-1-RR (PCM1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 84979-1-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84979-1-RR (PCM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 84979-1-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84979-1-RR (PCM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 84979-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PCM1 antibody (84979-1-RR, Clone: 242199A9 ) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of A549 using 84979-1-RR

  1x10^6 A549 cells were intracellularly stained with 0.25 ug PCM1 Recombinant antibody (84979-1-RR, Clone:242199A9) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of K562 using 84979-1-RR

  1x10^6 K562 were intracellularly stained with 0.25 ug PCM1 Recombinant antibody (84979-1-RR, Clone:242199A9) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 84979-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84979-1-RR against Human PCM1 were performed. The affinity constant is 21.0 nM.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A431, cellules HEK-293, cellules HT-1080, cellules NIH/3T3, cellules U-251
• Résultats positifs en IHC: tissu d'amygdalite humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,
• Résultats positifs en FC (Intra): K562, cellules A549

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:300-1:1200
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84979-1-RR cible PCM1 dans les applications de WB, IHC, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: PCM1 Protéine recombinante Ag13977
• Nom complet: pericentriolar material 1
• Masse moléculaire calculée: 2024 aa, 228 kDa
• Poids moléculaire observé: 245-320 kDa
• Numéro d’acquisition GenBank: BC000453
• Symbole du gène: PCM1
• Identification du gène (NCBI): 5108
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Pericentriolar material 1 (PCM1) is a component of centriolar satellites, which are electron dense granules scattered around centrosomes (PMID: 10579718). PCM1 is required for centrosome assembly and function. It acts as a scaffold to target several proteins to the centrosome in a dynein motor-dependent manner and regulate microtubular dynamics (PMID:18762586; 12403812). It is also involved in the biogenesis of cilia (PMID: 18772192). Chromosomal aberrations involving PCM1 gene are associated with papillary thyroid carcinomas and a variety of hematological malignancies, including atypical chronic myeloid leukemia and T-cell lymphoma. (PMID: 10980597; 16091753; 15805263; 16424865).

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