Anticorps Polyclonal de lapin anti-PCMT1

• Phare
• Validé par KD/KO

PCMT1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(9)

N° de cat : 10519-1-AP

Synonymes

L-isoaspartyl protein carboxyl methyltransferase, PCMT-1, PIMT, protein L isoaspartate (D aspartate) O methyltransferase, Protein L-isoaspartyl/D-aspartyl methyltransferase

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Galerie de données de validation

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  WB analysis using 10519-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10519-1-AP (PCMT1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10519-1-AP

  WB result of PCMT1 antibody (10519-1-AP, 1:1000) with si-Control and si-PCMT1 transfected HeLa cells.

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  WB analysis of HeLa using 10519-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10519-1-AP (PCMT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 10519-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 10519-1-AP (PCMT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10519-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10519-1-AP (PCMT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 10519-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 10519-1-AP (PCMT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas using 10519-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 10519-1-AP (PCMT1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human pancreas using 10519-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 10519-1-AP (PCMT1 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HEK-293 using 10519-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 10519-1-AP (PCMT1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: HeLa, cellules HEK-293, cellules HeLa, cellules Raji, tissu testiculaire de rat, tissu testiculaire de souris, tissu testiculaire humain
• Résultats positifs en IHC: tissu pancréatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HEK-293

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IHC: See 2 publications below

Informations sur le produit

10519-1-AP cible PCMT1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PCMT1 Protéine recombinante Ag0811
• Nom complet: protein-L-isoaspartate (D-aspartate) O-methyltransferase
• Masse moléculaire calculée: 25 kDa
• Poids moléculaire observé: 25-28 kDa
• Numéro d’acquisition GenBank: BC008748
• Symbole du gène: PCMT1
• Identification du gène (NCBI): 5110
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The PCMT1 gene encodes the protein repair enzyme protein-L-isoaspartate (D-aspartate) O-methyltransferase, which is known to protect certain neural cells against Bax-induced apoptosis.

Publications

Species	Application	Title
human	WB	Diabetologia; Post-translational protein modifications in type 1 diabetes: a role for the repair enzyme protein-L-isoaspartate (D-aspartate) O-methyltransferase?; Authors - W?gner A M AM
mouse	WB	Int J Cardiol; Protein-L-isoaspartate (D-aspartate) O-methyltransferase protects cardiomyocytes against hypoxia induced apoptosis through inhibiting proapoptotic kinase Mst1.; Authors - Yan Guijun G
human	WB	Front Cell Dev Biol; LINC00511/miRNA-143-3p Modulates Apoptosis and Malignant Phenotype of Bladder Carcinoma Cells via PCMT1.; Authors - Li-Ming Dong
human	WB	Biology (Basel); In Situ Peroxidase Labeling Followed by Mass-Spectrometry Reveals TIA1 Interactome.; Authors - Olga Gourdomichali
human	IHC	Transl Cancer Res; Elevated expression of protein-L-isoaspartate O-methyltransferase-1 (PCMT1) in cervical cancer; Authors - Liyun Shan
human	WB	J Exp Clin Cancer Res; Genome-wide CRISPR/Cas9 library screen identifies PCMT1 as a critical driver of ovarian cancer metastasis.; Authors - Jingjing Zhang ; KD Validated

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