Anticorps Polyclonal de lapin anti-PCNA

• Phare
• Validé par KD/KO

PCNA Polyclonal Antibody for FC (Intra), IF

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

IF, FC (Intra)

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

Publications

1

N° de cat : CL488-10205

Synonymes

Cyclin, PCNA

---

Galerie de données de validation

• 

  IF Staining of human breast cancer using CL488-10205

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite® Plus 488 PCNA antibody (CL488-10205) at dilution of 1:200.

• 

  FC experiment of Jurkat using CL488-10205

  1X10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human PCNA (CL488-10205) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

• 

  FC experiment of HeLa using CL488-10205

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human PCNA (CL488-10205) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

---

Applications testées

• Résultats positifs en IF: tissu de cancer du sein humain,
• Résultats positifs en cytométrie: cellules Jurkat, cellules HeLa

Dilution recommandée

• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 1 publications below

Informations sur le produit

CL488-10205 cible PCNA dans les applications de IF, FC (Intra) et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PCNA Protéine recombinante Ag0277
• Nom complet: proliferating cell nuclear antigen
• Masse moléculaire calculée: 29 kDa/31 kDa
• Poids moléculaire observé: 36-38 kDa
• Numéro d’acquisition GenBank: BC000491
• Symbole du gène: PCNA
• Identification du gène (NCBI): 5111
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Proliferating Cell Nuclear Antigen, commonly known as PCNA, is a protein that acts as a processivity factor for DNA polymerase δ in eukaryotic cells. This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. PCNA induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. It has to be loaded onto DNA in order to be able to stimulate APEX2. PCNA protein is highly conserved during evolution; the deduced amino acid sequences of rat and human differ by only 4 of 261 amino acids. PCNA has been used as loading control for proliferating cells. This antibody is a rabbit polyclonal antibody raised against an internal region of human PCNA. The calculated molecular weight of PCNA is 29 kDa, but modified PCNA is 36kDa (PMID: 1358458).

Publications

Species	Application	Title
human	IF	J Biol Chem; Aurora A-mediated pyruvate kinase M2 phosphorylation promotes biosynthesis with glycolytic metabolites and tumor cell cycle progression; Authors - Ya Jiang

Produits associés

Foxp3 / Transcription Factor Staining Buffer Kit

PF00011