Anticorps Recombinant de lapin anti-PCNA

PCNA Recombinant Antibody for IF-P

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

IF-P

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

240306B9

N° de cat : CL488-81302-6

Synonymes

proliferating cell nuclear antigen, Cyclin, 240306B9

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Galerie de données de validation

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  IF Staining of mouse testis using CL488-81302-6

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using CoraLite® Plus 488 PCNA antibody (CL488-81302-6, Clone: 240306B9 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse testis using CL488-81302-6

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using CoraLite® Plus 488 PCNA antibody (CL488-81302-6, Clone: 240306B9 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en IF-P: tissu testiculaire de souris,

Dilution recommandée

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-81302-6 cible PCNA dans les applications de IF-P et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: PCNA Protéine recombinante Ag0277
• Nom complet: proliferating cell nuclear antigen
• Masse moléculaire calculée: 29 kDa/31 kDa
• Poids moléculaire observé: 36 kDa
• Numéro d’acquisition GenBank: BC000491
• Symbole du gène: PCNA
• Identification du gène (NCBI): 5111
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Proliferating Cell Nuclear Antigen, commonly known as PCNA, is a protein that acts as a processivity factor for DNA polymerase δ in eukaryotic cells. This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. PCNA induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. It has to be loaded onto DNA in order to be able to stimulate APEX2. PCNA protein is highly conserved during evolution; the deduced amino acid sequences of rat and human differ by only 4 of 261 amino acids. PCNA has been used as loading control for proliferating cells. The calculated molecular weight of PCNA is 29 kDa, but modified PCNA is 36kDa (PMID: 1358458).

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