Anticorps Polyclonal de lapin anti-PCSK2

PCSK2 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(6)

N° de cat : 10553-1-AP

Synonymes

EC:3.4.21.94, KEX2-like endoprotease 2, NEC2, PC2, SPC2

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Galerie de données de validation

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  WB analysis using 10553-1-AP

  mouse brain tissue lysates were subjected to SDS PAGE followed by western blot with 10553-1-AP (PCSK2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 10553-1-AP

  mouse brain tissue lysates were subjected to SDS PAGE followed by western blot with 10553-1-AP (PCSK2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 10553-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10553-1-AP (PCSK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 10553-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 10553-1-AP (PCSK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris,
• Résultats positifs en IHC: tissu cérébral de souris, tissu cérébral de rat; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IF: See 2 publications below

Informations sur le produit

10553-1-AP cible PCSK2 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PCSK2 Protéine recombinante Ag0852
• Nom complet: proprotein convertase subtilisin/kexin type 2
• Masse moléculaire calculée: 70.5 kDa
• Poids moléculaire observé: 65-75 kDa
• Numéro d’acquisition GenBank: BC005815
• Symbole du gène: PCSK2
• Identification du gène (NCBI): 5126
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PCSK2(Prohormone convertase 2) is also named as NEC2(neuroendocrine convertase 2), PC2 and belongs to the peptidase S8 family. It is involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues. PCSK2 is responsible for the release of glucagon from proglucagon in pancreatic A cells. This protein has 3 isoforms produced by alternative splicing. PCSK2 is biosynthesized as a 74-kDa inactive precursor which is matured to a 64-kDa active enzyme by autocatalytic removal of its prodomain (PMID: 8397508, 21092739)

Publications

Species	Application	Title
human	IF	Nat Commun; Functional anterior pituitary generated in self-organizing culture of human embryonic stem cells.; Authors - Chikafumi Ozone
rat	WB	J Cell Biol; VAMP4 regulates insulin levels by targeting secretory granules to lysosomes; Authors - Min Li
mouse	WB	Biochem Biophys Res Commun; Elongator regulates the melanocortin satiety pathway.; Authors - Joseph Walters
rat	WB	Function (Oxf); ER Redox Homeostasis Regulates Proinsulin Trafficking and Insulin Granule Formation in the Pancreatic Islet β-Cell; Authors - Kristen E Rohli
mouse	IF	Front Mol Biosci; Novel cuproptosis metabolism-related molecular clusters and diagnostic signature for Alzheimer's disease; Authors - Fang Jia
		iScience; Insulin receptor isoform B is required for efficient proinsulin processing in pancreatic β cells; Authors - Mingchao Jiang

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