Anticorps Recombinant de lapin anti-PCSK9

PCSK9 Recombinant Antibody for IF/ICC, IF-P, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

IF/ICC, IF-P, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

230536E4

N° de cat : 83004-4-PBS

Synonymes

230536E4, AI415265, AI747682, FH3, HCHOLA3

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Galerie de données de validation

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  IF Staining of mouse liver using 83004-4-RR (same clone as 83004-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using PCSK9 antibody (83004-4-RR, Clone: 230536E4 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83004-4-PBS in a different storage buffer formulation.

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  IF Staining of mouse liver using 83004-4-RR (same clone as 83004-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using PCSK9 antibody (83004-4-RR, Clone: 230536E4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83004-4-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 83004-4-RR (same clone as 83004-4-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PCSK9 antibody (83004-4-RR, Clone: 230536E4 ) at dilution of 1:150 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 83004-4-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 83004-4-RR (same clone as 83004-4-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug PCSK9 Recombinant antibody (83004-4-RR, Clone:230536E4) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83004-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83004-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83004-4-RR against Mouse PCSK9 were performed. The affinity constant is 384 nM.

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Informations sur le produit

83004-4-PBS cible PCSK9 dans les applications de IF/ICC, IF-P, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: PCSK9 Protéine recombinante Eg0640
• Nom complet: proprotein convertase subtilisin/kexin type 9
• Masse moléculaire calculée: 75KD
• Numéro d’acquisition GenBank: NM_153565.2
• Symbole du gène: Pcsk9
• Identification du gène (NCBI): 100102
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PCSK9 (Proprotein convertase subtilisin/kexin type 9) is the ninth member of the proprotein convertase family that regulates lipoprotein homeostasis and altered PCSK9 expression was reportedly associated with tumor development and progression. PCSK9 is primarily synthesized and expressed in the liver but also in many other tissues, such as the intestinal tract, kidney, brain, heart, and blood vessels (PMID: 36242053).

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