Anticorps Recombinant de lapin anti-PCSK9

PCSK9 Recombinant Antibody for Sandwich ELISA, Indirect ELISA, Sample test

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

Sandwich ELISA, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

241395B7

N° de cat : 84172-6-PBS

Synonymes

EC:3.4.21.-, FH3, HCHOLA3, LDLCQ1, NARC 1

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Galerie de données de validation

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  Sandwich ELISA standard curve of MP01071-4

  Sandwich ELISA standard curve of MP01071-4, Human PCSK9 Recombinant Matched Antibody Pair - PBS only. 84172-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0362. 84172-6-PBS was HRP conjugated as the detection antibody. Range: 195-6250 pg/mL.

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  Sample test of MP01071-4

  Plasma of six individual healthy human donors was measured. The PCSK9 concentration of detected samples was determined to be 206.0 ng/mL with a range of ND-514.5 ng/mL.

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  Sample test of MP01071-4

  HepG2 were cultured in DMEM supplemented with 10% fetal bovine serum, 2.5 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernate was removed, assayed for human PCSK9, and measured 81.6 ng/mL.

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  Sample test of MP01071-4

  The mean PCSK9 concentration was determined to be 6.6 ng/mL in HepG2 cell extract based on a 2.0 mg/mL extract load.

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  Sample test of MP01071-4

  Serum of six individual healthy human donors was measured. The PCSK9 concentration of detected samples was determined to be 120.8 ng/mL with a range of ND-421.1 ng/mL.

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  Affinity and Kinetic Characterization of 84172-6-PBS

  Biolayer interferometry (BLl) kinetic assay of 84172-6-PBS against Human PCSK9 was performed. The affinity constant is 2.55 nM.

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Informations sur le produit

84172-6-PBS cible PCSK9 dans les applications de Sandwich ELISA, Indirect ELISA, Sample test et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: PCSK9 Protéine recombinante Eg0362
• Nom complet: proprotein convertase subtilisin/kexin type 9
• Masse moléculaire calculée: 74 kDa
• Numéro d’acquisition GenBank: NM_174936.4
• Symbole du gène: PCSK9
• Identification du gène (NCBI): 255738
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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