Anticorps Polyclonal de lapin anti-PCYT1A

PCYT1A Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

N° de cat : 30991-1-AP

Synonymes

CCT A, CCT alpha, CT A, CTPCT, PCYT1

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Galerie de données de validation

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  WB analysis using 30991-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30991-1-AP (PCYT1A antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 30991-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30991-1-AP (PCYT1A antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IF Staining of A431 using 30991-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using PCYT1A antibody (30991-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of U-251 using 30991-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U-251 cells using PCYT1A antibody (30991-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules L02, cellules Y79, tissu testiculaire de rat, tissu testiculaire de souris
• Résultats positifs en IF/ICC: cellules U-251, cellules A431

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

30991-1-AP cible PCYT1A dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PCYT1A Protéine recombinante Ag34268
• Nom complet: phosphate cytidylyltransferase 1, choline, alpha
• Masse moléculaire calculée: 42 kDa
• Poids moléculaire observé: 40-41 kDa
• Numéro d’acquisition GenBank: NM_005017
• Symbole du gène: PCYT1A
• Identification du gène (NCBI): 5130
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PCYT1A (phosphate cytidylyltransferase 1A, choline), also known as CTPCT. It is expected to be located in the nucleus and cytoplasm, which is enriched in brain, placenta, liver, fetal and adult lung. This gene belongs to the cytidylyltransferase family and is involved in the regulation of phosphatidylcholine biosynthesis. Mutations in this gene are associated with spondylometaphyseal dysplasia with cone-rod dystrophy. The molecular weight of PCYT1A is 41 kDa.

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