Anticorps Recombinant de lapin anti-PCYT1A

PCYT1A Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

230393B4

N° de cat : 83100-1-PBS

Synonymes

230393B4, CCT A, CCT alpha, CT A, CTPCT

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Galerie de données de validation

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  WB analysis using 83100-1-RR (same clone as 83100-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83100-1-RR (PCYT1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83100-1-PBS in a different storage buffer formulation.

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  WB analysis using 83100-1-RR (same clone as 83100-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83100-1-RR (PCYT1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83100-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 83100-1-RR (same clone as 83100-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using PCYT1A antibody (83100-1-RR, Clone: 230393B4 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 83100-1-PBS in a different storage buffer formulation.

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  FC experiment of A431 using 83100-1-RR (same clone as 83100-1-PBS)

  1x10^6 A431 cells were intracellularly stained with 0.25 ug Anti-Human PCYT1A (83100-1-RR, Clone:230393B4) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83100-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83100-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83100-1-RR against Human PCYT1A were performed. The affinity constant is below 1 pM.

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Informations sur le produit

83100-1-PBS cible PCYT1A dans les applications de WB, IF/ICC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: PCYT1A Protéine recombinante Ag34268
• Nom complet: phosphate cytidylyltransferase 1, choline, alpha
• Masse moléculaire calculée: 42 kDa
• Poids moléculaire observé: 38-41 kDa
• Numéro d’acquisition GenBank: NM_005017
• Symbole du gène: PCYT1A
• Identification du gène (NCBI): 5130
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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