Anticorps Polyclonal de lapin anti-PCYT2

• Phare
• Validé par KD/KO

PCYT2 Polyclonal Antibody for IF, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 14827-1-AP

Synonymes

ET, PCYT2

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Galerie de données de validation

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  WB analysis of human liver using 14827-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 14827-1-AP (PCYT2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 14827-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 14827-1-AP (PCYT2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 14827-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 14827-1-AP (PCYT2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 14827-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14827-1-AP (PCYT2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 14827-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 14827-1-AP (PCYT2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu hépatique humain, cellules HepG2, cellules MCF-7, tissu testiculaire de souris
• Résultats positifs en IF: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

14827-1-AP cible PCYT2 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PCYT2 Protéine recombinante Ag6616
• Nom complet: phosphate cytidylyltransferase 2, ethanolamine
• Masse moléculaire calculée: 44 kDa
• Poids moléculaire observé: 44 kDa
• Numéro d’acquisition GenBank: BC000351
• Symbole du gène: PCYT2
• Identification du gène (NCBI): 5833
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Ethanolamine-phosphate cytidylyltransferase (PCYT2) catalyzes the second step in the synthesis of phosphatidylethanolamine (PE) from ethanolamine via the CDP-ethanolamine pathway (PMID:9083101, PMID:31637422). Phosphatidylethanolamine is a dominant inner-leaflet phospholipid in cell membranes, where it plays a role in membrane function by structurally stabilizing membrane-anchored proteins, and participates in important cellular processes such as cell division, cell fusion, blood coagulation, and apoptosis (PMID:9083101).

Publications

Species	Application	Title
mouse	WB	Nature; Metabolic control of TFH cells and humoral immunity by phosphatidylethanolamine.; Authors - Guotong Fu ; KO Validated
human	IHC	Int J Mol Sci; Cancer Malignancy Is Correlated with Upregulation of PCYT2-Mediated Glycerol Phosphate Modification of α-Dystroglycan.; Authors - Fumiko Umezawa
human,mouse	IF,WB	Int J Biol Macromol; p75NTR enhances cognitive dysfunction in a mouse Alzheimer's disease model by inhibiting microRNA-210-3p-mediated PCYT2 through activation of NF-κB; Authors - Zhongliang Wei ; KD Validated
human	WB	Evid Based Complement Alternat Med; Metabolomic Study on Nude Mice Models of Gastric Cancer Treated with Modified Si Jun Zi Tang via HILIC UHPLC-Q-TOF/MS Analysis.; Authors - Shanshan Nie