Anticorps Recombinant de lapin anti-PD-1/CD279

PD-1/CD279 Recombinant Antibody for FC

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

FC

Conjugaison

Non conjugué

CloneNo.

EH12.2H7

N° de cat : 65586-1-MR

Synonymes

PD-1 (CD279), CD279, PD1, PD-1, PD 1

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Galerie de données de validation

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  FC experiment of human PBMCs using 65586-1-MR

  1x10^6 unstimulated or PHA stimulated human PBMCs were surface stained with 0.25 ug Anti-Human PD-1 (CD279) Mouse IgG2a Recombinant Antibody (65586-1-MR, Clone: EH12.2H7) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD3. Cells were not fixed.

• 

  FC experiment of human PBMCs using 65586-1-MR

  1x10^6 unstimulated (black) or PHA stimulated human PBMCs (red) were surface stained with 0.25 ug Anti-Human PD-1 (CD279) Mouse IgG2a Recombinant Antibody (65586-1-MR, Clone: EH12.2H7) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). 1x10^6 PHA treated human PBMCs were surface stained with Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) (blue). Cells were not fixed.

• 

  FC experiment of human PBMCs using 65586-1-MR

  1x10^6 PHA stimulated human PBMCs were surface stained with 0.25 ug Anti-Human PD-1 (CD279) Mouse IgG2a Recombinant Antibody (65586-1-MR, Clone: EH12.2H7) (left) or 0.25 ug Anti-Human PD-1/CD279 (65168-1-Ig, Clone: EH12.2H7) (right) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD3. Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: PHA treated human PBMCs,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

65586-1-MR cible PD-1/CD279 dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: s/o
• Nom complet: programmed cell death 1
• Masse moléculaire calculée: 288 aa, 32 kDa
• Numéro d’acquisition GenBank: BC074740
• Symbole du gène: PD-1
• Identification du gène (NCBI): 5133
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2-8°C. Stable for one year after shipment.

Informations générales

Programmed cell death 1 (PD-1, also known as CD279) is an immunoinhibitory receptor that belongs to the CD28/CTLA-4 subfamily of the Ig superfamily. It is a 288 amino acid (aa) type I transmembrane protein composed of one Ig superfamily domain, a stalk, a transmembrane domain, and an intracellular domain containing an immunoreceptor tyrosine-based inhibitory motif (ITIM) as well as an immunoreceptor tyrosine-based switch motif (ITSM) (PMID: 18173375). PD-1 is expressed during thymic development and is induced in a variety of hematopoietic cells in the periphery by antigen receptor signaling and cytokines (PMID: 20636820). Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function (PMID: 19426218). It is critical for the regulation of T cell function during immunity and tolerance. Blockade of PD-1 can overcome immune resistance and also has been shown to have antitumor activity (PMID: 22658127; 23169436).

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