Anticorps Recombinant de lapin anti-PD-1/CD279

PD-1/CD279 Recombinant Antibody for Sandwich ELISA, Indirect ELISA, Sample test

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

Sandwich ELISA, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

240389D12

N° de cat : 83516-9-PBS

Synonymes

CD279, PD-1, PD1, Pdc1, PD 1

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Galerie de données de validation

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  Sandwich ELISA standard curve of MP00496-4

  Sandwich ELISA standard curve of MP00496-4, Mouse PD-1/CD279 Monoclonal Matched Antibody Pair - PBS only. 83516-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0918. 83516-9-PBS was HRP conjugated as the detection antibody. Range: 62.5-4000 pg/mL.

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  Sample test of MP00496-4

  The mean mouse PD-1/CD279 concentration was determined to be 14.08 ng/mL in EL-4 cell extract based on a 3.0 mg/mL extract load.

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  Sample test of MP00496-4

  EL-4 was cultured in RPMI 1640 supplemented with 10% fetal bovine serum, 50 μM β-mercaptoethanol, 2 mM L-glutamine, and 100 μg/mL ofstreptomycin sulfate.The cellswere stimulated with 10 μg/mL of PHA and 10 ng/mL of PMA. An aliquot of the cell culture supernatant wasremoved, assayed for mouse PD-1/CD279 and measured 812.8 pg/mL.

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Informations sur le produit

83516-9-PBS cible PD-1/CD279 dans les applications de Sandwich ELISA, Indirect ELISA, Sample test et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: programmed cell death 1
• Masse moléculaire calculée: 32 kDa
• Numéro d’acquisition GenBank: NM_008798.2
• Symbole du gène: PD-1
• Identification du gène (NCBI): 18566
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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