Anticorps Recombinant de lapin anti-PD-1/CD279

PD-1/CD279 Recombinant Antibody for FC

Hôte / Isotype

Rat / IgG2a

Réactivité testée

souris

Applications

FC

Conjugaison

Non conjugué

CloneNo.

29F.1A12

N° de cat : 98080-7-TR

Synonymes

CD279, PD1, PD-1, PD 1, Pdc1

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Galerie de données de validation

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  FC experiment of mouse splenocytes using 98080-7-TR

  1x10^6 untreated or anti-CD3/CD28 treated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse PD-1/CD279 Rat RecAb (98080-6-TR, Clone:29F.1A12) and PE anti-rat IgG2a Antibody. Cells were then stained with CoraLite® Plus 647 Anti-Mouse CD3. Cells were not fixed.

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  FC experiment of mouse splenocytes using 98080-7-TR

  1x10^6 anti-CD3/CD28 treated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse PD-1/CD279 Rat RecAb (98080-6-TR, Clone: 29F.1A12) (red) or Rat IgG2a Isotype Control (2A3) (65209-1-Ig, Clone: 2A3) (blue), and PE anti-rat IgG2a Antibody. 1x10^6 untreated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse PD-1/CD279 Rat RecAb (98080-6-TR, Clone: 29F.1A12) (black, dashed) and PE anti-rat IgG2a Antibody. Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: Anti-CD3/CD28 treated mouse splenocytes

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

98080-7-TR cible PD-1/CD279 dans les applications de FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Rat / IgG2a
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: unknow
• Nom complet: programmed cell death 1
• Symbole du gène: PD-1
• Identification du gène (NCBI): 18566
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2 - 8°C. Stable for one year after shipment.

Informations générales

Programmed cell death 1 (PD-1, also known as CD279) is an immunoinhibitory receptor that belongs to the CD28/CTLA-4 subfamily of the Ig superfamily. It is a 288 amino acid (aa) type I transmembrane protein composed of one Ig superfamily domain, a stalk, a transmembrane domain, and an intracellular domain containing an immunoreceptor tyrosine-based inhibitory motif (ITIM) as well as an immunoreceptor tyrosine-based switch motif (ITSM) (PMID: 18173375). PD-1 is expressed during thymic development and is induced in a variety of hematopoietic cells in the periphery by antigen receptor signaling and cytokines (PMID: 20636820). Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function (PMID: 19426218). It is critical for the regulation of T cell function during immunity and tolerance. Blockade of PD-1 can overcome immune resistance and also has been shown to have antitumor activity (PMID: 22658127; 23169436). RMP1-14 is a monoclonal antibody that targets the murine PD-1 protein and can be used in mouse models to block the reaction between PD-1 and PD-L1/PD-L2 (PMID: 14764726; 31832706).

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