Anticorps Monoclonal anti-PD-1/CD279

• Phare
• Validé par KD/KO

PD-1/CD279 Monoclonal Antibody for IF-P

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat, souris

Applications

IF-P

Conjugaison

CoraLite®594 Fluorescent Dye

CloneNo.

4H4D1

N° de cat : CL594-66220

Synonymes

CD279, PD1, PD-1, 4H4D1, PD 1

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Galerie de données de validation

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  IF Staining of human lymphoma using CL594-66220

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human angioimmunoblastic T-cell lymphoma tissue using CoraLite®594 PD-1/CD279 antibody (CL594-66220, Clone: 4H4D1 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human lymphoma using CL594-66220

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human angioimmunoblastic T-cell lymphoma tissue using CoraLite®594 PD-1/CD279 antibody (CL594-66220, Clone: 4H4D1 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en IF-P: tissu de lymphome humain,

Dilution recommandée

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL594-66220 cible PD-1/CD279 dans les applications de IF-P et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PD-1/CD279 Protéine recombinante Ag12470
• Nom complet: programmed cell death 1
• Masse moléculaire calculée: 288 aa, 32 kDa
• Poids moléculaire observé: 32 kDa, 47-55 kDa
• Numéro d’acquisition GenBank: BC074740
• Symbole du gène: PD-1
• Identification du gène (NCBI): 5133
• Conjugaison: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Programmed cell death 1 (PD-1, also known as CD279) is an immunoinhibitory receptor that belongs to the CD28/CTLA-4 subfamily of the Ig superfamily. It is a 288 amino acid (aa) type I transmembrane protein composed of one Ig superfamily domain, a stalk, a transmembrane domain, and an intracellular domain containing an immunoreceptor tyrosine-based inhibitory motif (ITIM) as well as an immunoreceptor tyrosine-based switch motif (ITSM) (PMID: 18173375). PD-1 is expressed during thymic development and is induced in a variety of hematopoietic cells in the periphery by antigen receptor signaling and cytokines (PMID: 20636820). Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function (PMID: 19426218). It is critical for the regulation of T cell function during immunity and tolerance. Blockade of PD-1 can overcome immune resistance and also has been shown to have antitumor activity (PMID: 22658127; 23169436). The calculated molecular weight of PD-1 is 32 kDa. It has been reported that PD-1 is heavily glycosylated and migrates with an apparent molecular mass of 47-55 kDa on SDS-PAGE (PMID: 8671665; 17640856; 17003438).

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