Anticorps Recombinant de lapin anti-PD-1/CD279

PD-1/CD279 Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

FC

Conjugaison

CoraLite®594 Fluorescent Dye

CloneNo.

240389B8

N° de cat : CL594-98080

Synonymes

CD279, PD-1, PD1, Pdc1, PD 1

---

Galerie de données de validation

• 

  FC experiment of mouse splenocytes using CL594-98080

  1x10^6 untreated or anti-CD3/CD28 treated mouse splenocytes were surface stained with FITC Plus Anti-Mouse CD3 and 0.25 ug CoraLite®594 Anti-Mouse PD-1/CD279 Rabbit RecAb (CL594-98080, Clone:240389B8). Cells were not fixed.

• 

  FC experiment of mouse splenocytes using CL594-98080

  1x10^6 anti-CD3/CD28 treated mouse splenocytes were surface stained with 0.25 ug CoraLite®594 Anti-Mouse PD-1/CD279 Rabbit RecAb (CL594-98080, Clone:240389B8) (red) or CoraLite®594 Rabbit IgG Isotype Control RecAb (CL594-98136, Clone: 240953C9) (blue). 1x10^6 untreated mouse splenocytes were surface stained with 0.25 ug CoraLite®594 Anti-Mouse PD-1/CD279 Rabbit RecAb (CL594-98080, Clone:240389B8) (black, dashed). Cells were not fixed.

---

Applications testées

• Résultats positifs en cytométrie: anti-CD3/CD28 treated mouse splenocytes,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL594-98080 cible PD-1/CD279 dans les applications de FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: programmed cell death 1
• Masse moléculaire calculée: 32 kDa
• Numéro d’acquisition GenBank: NM_008798.2
• Symbole du gène: PD-1
• Identification du gène (NCBI): 18566
• Conjugaison: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

Programmed cell death 1 (PD-1, also known as CD279) is an immunoinhibitory receptor that belongs to the CD28/CTLA-4 subfamily of the Ig superfamily. It is a 288 amino acid (aa) type I transmembrane protein composed of one Ig superfamily domain, a stalk, a transmembrane domain, and an intracellular domain containing an immunoreceptor tyrosine-based inhibitory motif (ITIM) as well as an immunoreceptor tyrosine-based switch motif (ITSM) (PMID: 18173375). PD-1 is expressed during thymic development and is induced in a variety of hematopoietic cells in the periphery by antigen receptor signaling and cytokines (PMID: 20636820). Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function (PMID: 19426218). It is critical for the regulation of T cell function during immunity and tolerance. Blockade of PD-1 can overcome immune resistance and also has been shown to have antitumor activity (PMID: 22658127; 23169436).

{{ptg:RelatedPrimaryAntibodies}}