Anticorps Monoclonal anti-PD-1/CD279

PD-1/CD279 Monoclonal Antibody for FC

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

FC

Conjugaison

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

J110

N° de cat : CL750-65119

Synonymes

CD279, PD1, PD-1, hPD 1, hPD l

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Galerie de données de validation

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  FC experiment of human PBMCs using CL750-65119

  1x10^6 untreated (center) or PHA treated (3 day) human PBMCs were surface stained with PE Anti-Human CD3 (PE-65151, Clone:UCHT1) and 5 ul CoraLite® Plus 750 Anti-Human PD-1/CD279 (CL750-65119, Clone:J110) (right) or CoraLite®750 Mouse IgG1 Isotype Control (CL750-65124, Clone: MOPC-21) (left). Cells were not fixed. Cells were treated with FC Receptor Block prior to staining. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using CL750-65119

  1x10^6 untreated (black dashed) or PHA treated (3 day) human PBMCs were surface stained with 5 ul CoraLite® Plus 750 Anti-Human PD-1/CD279 (CL750-65119, Clone:J110) (red) or CoraLite®750 Mouse IgG1 Isotype Control (CL750-65124, Clone: MOPC-21) (blue). Cells were not fixed. Cells were treated with FC Receptor Block prior to staining. Lymphocytes were gated.

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Applications testées

• Résultats positifs en cytométrie: PHA treated human PBMCs,

Dilution recommandée

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL750-65119 cible PD-1/CD279 dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: s/o
• Nom complet: programmed cell death 1
• Masse moléculaire calculée: 288 aa, 32 kDa
• Numéro d’acquisition GenBank: BC074740
• Symbole du gène: PD-1
• Identification du gène (NCBI): 5133
• Conjugaison: CoraLite® Plus 750 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 755 nm / 780 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

Programmed cell death 1 (PD-1, also known as CD279) is an immunoinhibitory receptor that belongs to the CD28/CTLA-4 subfamily of the Ig superfamily. It is a 288 amino acid (aa) type I transmembrane protein composed of one Ig superfamily domain, a stalk, a transmembrane domain, and an intracellular domain containing an immunoreceptor tyrosine-based inhibitory motif (ITIM) as well as an immunoreceptor tyrosine-based switch motif (ITSM) (PMID: 18173375). PD-1 is expressed during thymic development and is induced in a variety of hematopoietic cells in the periphery by antigen receptor signaling and cytokines (PMID: 20636820). Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function (PMID: 19426218). It is critical for the regulation of T cell function during immunity and tolerance. Blockade of PD-1 can overcome immune resistance and also has been shown to have antitumor activity (PMID: 22658127; 23169436).

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