Anticorps Monoclonal anti-PD-L1/CD274

PD-L1/CD274 Monoclonal Antibody for FC

Hôte / Isotype

Mouse / IgG2b, kappa

Réactivité testée

Humain

Applications

IHC, IF, FC

Conjugaison

APC Fluorescent Dye

Publications(11)

CloneNo.

29E.2A3

N° de cat : APC-65081

Synonymes

CD274, PD-L1, 29E.2A3, B7 H1, B7-H1

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Galerie de données de validation

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  FC experiment of human peripheral blood lymphocytes using APC-65081

  1X10^6 PHA-stimulated (5 ug/ml, 3 days) human peripheral blood lymphocytes were surface stained with 5 ul APC Anti-Human PD-L1 (B7-H1) (APC-65081, Clone: 29E.2A3) (blue) or isotype control antibody (black). Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: lymphocytes de sang périphérique humain,

Dilution recommandée

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 1 publications below
• IF: See 1 publications below
• FC: See 8 publications below

Informations sur le produit

APC-65081 cible PD-L1/CD274 dans les applications de IHC, IF, FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG2b, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PD-L1 humain pleine longueur
• Nom complet: CD274 molecule
• Numéro d’acquisition GenBank: BC069381
• Symbole du gène: PD-L1
• Identification du gène (NCBI): 29126
• Conjugaison: APC Fluorescent Dye
• Excitation/Emission maxima wavelengths: 650 nm / 660 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS with 0.09% sodium azide and 0.1% gelatin
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

Programmed cell death ligand 1 (PD-L1, CD274, or B7-H1), is the first member of B7 family to be discovered. B7 family molecules are type I transmembrane proteins belonging to the immunoglobulin superfamily. In concert with their CD28 family receptors, the B7s are key regulators of the adaptive immune response. PD-L1 is suggested as a negative regulator of T and B cell, and plays important role in mediating tolerance of lymphocytes to self-antigens. It is also involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PD-1-independent manner.

Publications

Species	Application	Title
	FC	Adv Sci (Weinh); Inhibition of CK2/ING4 Pathway Facilitates Non-Small Cell Lung Cancer Immunotherapy; Authors - Qian Gou
		J Immunother Cancer; eEF2K promotes PD-L1 stabilization through inactivating GSK3β in melanoma.; Authors - Xisha Chen
human	FC	Oncogene; Dual role of pseudogene TMEM198B in promoting lipid metabolism and immune escape of glioma cells; Authors - Ying Zhan
human	FC	iScience; Cancer-associated fibroblasts promote enzalutamide resistance and PD-L1 expression in prostate cancer through CCL5-CCR5 paracrine axis; Authors - Zhi Xiong
		Cancer Sci; PPARγ inhibited tumor immune escape by inducing PD-L1 autophagic degradation; Authors - Qian Gou
human	FC	Stem Cell Res Ther; Glioma-associated mesenchymal stem cells-mediated PD-L1 expression is attenuated by Ad5-Ki67/IL-15 in GBM treatment.; Authors - Qing Zhang

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