Anticorps Recombinant de lapin anti-PD-L1/CD274

PD-L1/CD274 Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

240993C9

N° de cat : 83906-6-PBS

Synonymes

Cd274, PD-L1, 240993C9, B7 H1, PD L1

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Galerie de données de validation

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  WB analysis using 83906-6-RR (same clone as 83906-6-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83906-6-RR (PD-L1/CD274 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83906-6-PBS in a different storage buffer formulation.

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  IHC staining of mouse spleen using 83906-6-RR (same clone as 83906-6-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83906-6-RR (PD-L1/B7-H1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83906-6-PBS in a different storage buffer formulation.

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  IHC staining of mouse spleen using 83906-6-RR (same clone as 83906-6-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83906-6-RR (PD-L1/B7-H1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83906-6-PBS in a different storage buffer formulation.

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  IF Staining of human placenta using 83906-6-RR (same clone as 83906-6-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using PD-L1/CD274 antibody (83906-6-RR, Clone: 240993C9 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83906-6-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83906-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83906-6-RR against Mouse PD-L1/B7-H1 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

83906-6-PBS cible PD-L1/CD274 dans les applications de WB, IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: PD-L1/CD274 Protéine recombinante Eg0986
• Nom complet: CD274 antigen
• Masse moléculaire calculée: 33 kDa
• Poids moléculaire observé: 45 kDa
• Numéro d’acquisition GenBank: NM_021893.3
• Symbole du gène: PD-L1
• Identification du gène (NCBI): 60533
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Programmed cell death ligand 1 (PD-L1, CD274, or B7-H1), is the first member of B7 family to be discovered. B7 family molecules are type I transmembrane proteins belonging to the immunoglobulin superfamily. In concert with their CD28 family receptors, the B7s are key regulators of the adaptive immune response. PD-L1 is suggested as a negative regulator of T and B cell, and plays important role in mediating tolerance of lymphocytes to self-antigens. It is also involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PD-1-independent manner.

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