Anticorps Recombinant de lapin anti-PD-L1/CD274

PD-L1/CD274 Recombinant Antibody for FC

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

souris

Applications

FC

Conjugaison

Non conjugué

CloneNo.

10F.9G2

N° de cat : 65685-1-MR

Synonymes

Cd274, PD-L1, B7 H1, PD L1, Pdcd1l1

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Galerie de données de validation

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  FC experiment of mouse splenocytes using 65685-1-MR

  1x10^6 untreated or anti-CD3/CD28 treated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse PD-L1/CD274 (10F.9G2) Mouse IgG2a RecAb (65685-1-MR, Clone:10F.9G2) and PE-conjugated Goat Anti-Mouse IgG. Cells were co-stained with APC Anti-Mouse CD3. Cells were not fixed.

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  FC experiment of mouse splenocytes using 65685-1-MR

  1x10^6 anti-CD3/CD28 treated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse PD-L1/CD274 (10F.9G2) Mouse IgG2a RecAb (65685-1-MR, Clone: 10F.9G2) (red) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue) and PE-conjugated Goat Anti-Mouse IgG. 1x10^6 untreated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse PD-L1/CD274 (10F.9G2) Mouse IgG2a RecAb (65685-1-MR, Clone:10F.9G2) (black, dashed) and PE-conjugated Goat Anti-Mouse IgG. Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: Anti-CD3/CD28 treated mouse splenocytes

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

65685-1-MR cible PD-L1/CD274 dans les applications de FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: s/o
• Nom complet: CD274 antigen
• Numéro d’acquisition GenBank: BC066841
• Symbole du gène: PD-L1
• Identification du gène (NCBI): 60533
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2-8°C. Stable for one year after shipment.

Informations générales

Programmed cell death ligand 1 (PD-L1, CD274, or B7-H1), is the first member of B7 family to be discovered. B7 family molecules are type I transmembrane proteins belonging to the immunoglobulin superfamily. In concert with their CD28 family receptors, the B7s are key regulators of the adaptive immune response. PD-L1 is suggested as a negative regulator of T and B cell, and plays important role in mediating tolerance of lymphocytes to self-antigens. It is also involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PD-1-independent manner.

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