Anticorps Recombinant de lapin anti-PD-L1/CD274

PD-L1/CD274 Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC

Conjugaison

Non conjugué

CloneNo.

240721H8

N° de cat : 98062-1-PBS

Synonymes

CD274, PD-L1, PD L1, hPD-L1, B7-H1

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Galerie de données de validation

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  FC experiment of human PBMCs using 98062-1-RR (same clone as 98062-1-PBS)

  1x10^6 unstimulated or PHA stimulated human PBMCs were stained with 0.25 ug Anti-Human PD-L1/CD274 Rabbit Recombinant Antibody (98062-1-RR, Clone:240721H8) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Cells were then stained with CoraLite® Plus 647 Anti-Human CD3 (OKT3) Mouse IgG2a Recombinant Antibody (CL647-65569, Clone: OKT3). Cells were not fixed. This data was developed using the same antibody clone with 98062-1-PBS in a different storage buffer formulation.

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  FC experiment of human PBMCs using 98062-1-RR (same clone as 98062-1-PBS)

  1x10^6 unstimulated (black) or PHA stimulated human PBMCs (red) were stained with 0.25 ug Anti-Human PD-L1/CD274 Rabbit Recombinant Antibody (98062-1-RR, Clone:240721H8) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), 1x10^6 PHA treated human PBMCs were stained with Isotype Control(blue). Cells were not fixed. This data was developed using the same antibody clone with 98062-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 98062-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98062-1-RR against Human PD-L1/CD274 were performed. The affinity constant is 0.558 nM.

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Informations sur le produit

98062-1-PBS cible PD-L1/CD274 dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: CD274 molecule
• Masse moléculaire calculée: 290 aa, 33 kDa
• Numéro d’acquisition GenBank: BC074984
• Symbole du gène: PD-L1
• Identification du gène (NCBI): 29126
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C.

Informations générales

Programmed cell death ligand 1 (PD-L1, CD274, or B7-H1), is the first member of B7 family to be discovered. B7 family molecules are type I transmembrane proteins belonging to the immunoglobulin superfamily. In concert with their CD28 family receptors, the B7s are key regulators of the adaptive immune response. PD-L1 is suggested as a negative regulator of T and B cell, and plays important role in mediating tolerance of lymphocytes to self-antigens. It is also involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PD-1-independent manner.

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