Anticorps Recombinant de lapin anti-PD-L1/CD274

PD-L1/CD274 Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC

Conjugaison

Non conjugué

CloneNo.

240721H8

N° de cat : 98062-1-RR

Synonymes

CD274, PD-L1, PD L1, hPD-L1, B7-H1

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Galerie de données de validation

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  FC experiment of human PBMCs using 98062-1-RR

  1x10^6 unstimulated or PHA stimulated human PBMCs were stained with 0.25 ug Anti-Human PD-L1/CD274 Rabbit Recombinant Antibody (98062-1-RR, Clone:240721H8) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Cells were then stained with CoraLite® Plus 647 Anti-Human CD3 (OKT3) Mouse IgG2a Recombinant Antibody (CL647-65569, Clone: OKT3). Cells were not fixed.

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  FC experiment of human PBMCs using 98062-1-RR

  1x10^6 unstimulated (black) or PHA stimulated human PBMCs (red) were stained with 0.25 ug Anti-Human PD-L1/CD274 Rabbit Recombinant Antibody (98062-1-RR, Clone:240721H8) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), 1x10^6 PHA treated human PBMCs were stained with Isotype Control(blue). Cells were not fixed.

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  Affinity and Kinetic Characterization of 98062-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98062-1-RR against Human PD-L1/CD274 were performed. The affinity constant is 0.558 nM.

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Applications testées

• Résultats positifs en cytométrie: PHA treated human PBMCs,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

98062-1-RR cible PD-L1/CD274 dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: CD274 molecule
• Masse moléculaire calculée: 290 aa, 33 kDa
• Numéro d’acquisition GenBank: BC074984
• Symbole du gène: PD-L1
• Identification du gène (NCBI): 29126
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2 - 8°C. Stable for one year after shipment.

Informations générales

Programmed cell death ligand 1 (PD-L1, CD274, or B7-H1), is the first member of B7 family to be discovered. B7 family molecules are type I transmembrane proteins belonging to the immunoglobulin superfamily. In concert with their CD28 family receptors, the B7s are key regulators of the adaptive immune response. PD-L1 is suggested as a negative regulator of T and B cell, and plays important role in mediating tolerance of lymphocytes to self-antigens. It is also involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PD-1-independent manner.

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