Anticorps Recombinant de lapin anti-PD-L1/CD274

PD-L1/CD274 Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC

Conjugaison

FITC Plus Fluorescent Dye

CloneNo.

240721H8

N° de cat : FITC-98062

Synonymes

CD274, PD-L1, 240721H8, B7 H1, B7-H1

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Galerie de données de validation

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  FC experiment of human PBMCs using FITC-98062

  1x10^6 untreated or PHA-treated human PBMCs were surface stained with APC Anti-Human CD3 and 5 ul FITC Plus Anti-Human PD-L1/CD274 Rabbit RecAb (FITC-98062, Clone: 240721H8). Cells were not fixed.

• 

  FC experiment of human PBMCs using FITC-98062

  1x10^6 PHA-treated human PBMCs were surface stained with 5 ul FITC Plus Anti-Human PD-L1/CD274 Rabbit RecAb (FITC-98062, Clone: 240721H8) (red) or FITC Plus Rabbit IgG Isotype Control RecAb (FITC-98136, Clone: 240953C9) (blue). Untreated human PBMCs were surface stained with 5 ul FITC Plus Anti-Human PD-L1/CD274 Rabbit RecAb (FITC-98062, Clone:240721H8) (black, dashed). Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: PHA treated human PBMCs,

Dilution recommandée

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Informations sur le produit

FITC-98062 cible PD-L1/CD274 dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: PD-L1/CD274 Protéine recombinante Eg1114
• Nom complet: CD274 molecule
• Masse moléculaire calculée: 290 aa, 33 kDa
• Numéro d’acquisition GenBank: BC074984
• Symbole du gène: PD-L1
• Identification du gène (NCBI): 29126
• Conjugaison: FITC Plus Fluorescent Dye
• Excitation/Emission maxima wavelengths: 495 nm / 524 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

Programmed cell death ligand 1 (PD-L1, CD274, or B7-H1), is the first member of B7 family to be discovered. B7 family molecules are type I transmembrane proteins belonging to the immunoglobulin superfamily. In concert with their CD28 family receptors, the B7s are key regulators of the adaptive immune response. PD-L1 is suggested as a negative regulator of T and B cell, and plays important role in mediating tolerance of lymphocytes to self-antigens. It is also involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PD-1-independent manner.

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