Anticorps Polyclonal de lapin anti-PD-L2/CD273

PD-L2/CD273 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 27406-1-PBS

Synonymes

PDCD1LG2/PD-L2, PDCD1LG2, PD-L2, B7 DC, B7DC

---

Galerie de données de validation

• 

  WB analysis using 27406-1-AP (same clone as 27406-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 27406-1-AP (PDCD1LG2/PD-L2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27406-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 27406-1-AP (same clone as 27406-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 27406-1-AP (PD-L2/CD273 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27406-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 27406-1-AP (same clone as 27406-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 27406-1-AP (PD-L2/CD273 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27406-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat heart using 27406-1-AP (same clone as 27406-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 27406-1-AP (PD-L2/CD273 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27406-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat heart using 27406-1-AP (same clone as 27406-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 27406-1-AP (PD-L2/CD273 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27406-1-PBS in a different storage buffer formulation.

• 

  IF Staining of NIH/3T3 using 27406-1-AP (same clone as 27406-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using PDCD1LG2/PD-L2 antibody (27406-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 27406-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

27406-1-PBS cible PD-L2/CD273 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PD-L2/CD273 Protéine recombinante Ag26384
• Nom complet: programmed cell death 1 ligand 2
• Masse moléculaire calculée: 273 aa, 31 kDa
• Poids moléculaire observé: 45 kDa
• Numéro d’acquisition GenBank: BC074766
• Symbole du gène: PD-L2
• Identification du gène (NCBI): 80380
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Programmed cell death 1 ligand 2 (PDCD1LG2), also known as PD-L2, CD273 and B7-DC, is an immune checkpoint receptor ligand which plays a role in negative regulation of the adaptive immune response. PDCD1LG2/PD-L2 protein is expressed mainly by dendritic cells, macrophages, and tumor cells, and down-regulates the effector functions of T cells via PD-1/PD-L2 axis in the tumor microenvironment (PMID: 30275216). The endogenous PDCD1LG2/PD-L2 protein displays a molecular weight of ∼45 kDa(glycosylated form), and a minor band at 35 kDa (non-glycosylated form) in western blot (PMID: 28811964, PMID: 34697216).

{{ptg:RelatedPrimaryAntibodies}}