Anticorps Monoclonal anti-PDCD4

• Phare
• Validé par KD/KO

PDCD4 Monoclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

3

CloneNo.

1F1A11

N° de cat : 66100-1-Ig

Synonymes

H731, Nuclear antigen H731 like, PDCD4, Protein 197/15a

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Galerie de données de validation

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  WB analysis of HEK293 cells using 66100-1-Ig

  WB result of PDCD4 antibody (66100-1-Ig, 1:4000) with si-control and si-PDCD4 transfected HEK293 cells.

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  WB analysis of HeLa using 66100-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66100-1-Ig (PDCD4 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66100-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66100-1-Ig (PDCD4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 66100-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66100-1-Ig (PDCD4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 66100-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66100-1-Ig (PDCD4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 66100-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66100-1-Ig (PDCD4 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 66100-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66100-1-Ig (PDCD4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 66100-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66100-1-Ig (PDCD4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK293
• Résultats positifs en IHC: tissu de cancer du foie humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below

Informations sur le produit

66100-1-Ig cible PDCD4 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PDCD4 Protéine recombinante Ag19239
• Nom complet: programmed cell death 4 (neoplastic transformation inhibitor)
• Masse moléculaire calculée: 469 aa, 52 kDa
• Poids moléculaire observé: 56 kDa
• Numéro d’acquisition GenBank: BC026104
• Symbole du gène: PDCD4
• Identification du gène (NCBI): 27250
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Programmed cell death 4 (Pdcd4) is a novel tumor suppressor that inhibits translation, progression and invasion. It was first identified as being differnetially upregulated during apoptosis. Pdcd4 interferes with the activity of the eukaryotic initiation factor (eIF) 4A by displacing the scaffold protein eIF4G from its binding to the RNA helicase eIF4A.

Publications

Species	Application	Title
human	WB	J Med Chem; Discovery of a Novel Small-Molecule Inhibitor Disrupting TRBP-Dicer Interaction against Hepatocellular Carcinoma via the Modulation of microRNA Biogenesis.; Authors - Ting Peng
human	WB	Clin Res Hepatol Gastroenterol; Long non-coding RNA LINC00472 suppresses hepatocellular carcinoma cell proliferation, migration and invasion through miR-93-5p/PDCD4 pathway.; Authors - Changyu Chen
human	WB	J Tradit Chin Med; Qilan preparation inhibits proliferation and induces apoptosis by down-regulating microRNA-21 in human Tca8113 tongue squamous cell carcinoma cells; Authors - Ding Jiamin